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DARMAWAN SAPTADI
Plant Breeding Laboratory, Department of Agronomy, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers DARMAWAN SAPTADI; NUR ASBANI; BAMBANG HELIYANTO; ASEP SETIAWAN; SUDARSONO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 2 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210249

Abstract

Abstract. Saptadi D, Asbani N, Heliyanto B, Setiawan A, Sudarsono. 2020. Identification of interspecific hybrid between Jatropha curcas x J. integerrima using morphological and molecular markers. Biodiversitas 21: 814-823. Eight F1 progenies derived from Jatropha curcas × J. integerrima hybridizations were evaluated for their morphological characters and using RAPD, ISSR and SSR markers. Morphological variations among the hybrids were limited and they were intermediate between the Jatropha parents. The eight F1 progenies derived from J. curcas × J. integerrima hybridizations were most probably the interspecific F1 hybrids. The confirmed identity of the progenies as interspecific hybrids between J. curcas × J. integerrima was based on the presence of several phenotypic characters from both parents in the F1 progenies and by similarity of the molecular marker banding patterns among the parents and the F1 progenies. Among the evaluated molecular markers, the ISSR primers and the majority of either RAPD and SSR primers were not able to generate marker for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. However, the RAPD primer OPC 10 and the SSR primers AF469003, EU099522 and EU586348 were able to generate polymorphic markers in the Jatropha parents and their F1 progenies. Therefore, these four primers were able to generate usable markers for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. The evaluated interspecific F1 progenies are potentially useful to increase genetic diversity of J. curcas and support its breeding program.
Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha Darmawan Saptadi; Bambang Heliyanto; Sudarsono Sudarsono
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211111

Abstract

Abstract. Saptadi D, Heliyanto B, Sudarsono. 2020. Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha. Biodiversitas 21: 5072-5076. The transferability of SSR markers can be used to access the genetic diversity of related species. There are four close relatives of Jatropha curcas L in Indonesia, which can be utilized as a new diversity source through the interspecific crossing. This research was conducted to determine the ability of cross-species amplification of SSR markers developed from J. curcas to other Jatropha species (J. integerrima, J. multifida, J. gossypifolia, J. podagrica). It also investigated the relationship between these species. Out of 28 primers checked,11 primers showed cross-species amplification in all the species tested. Primer pairs EU099519, EU099528, and EU099525 have no transferability to other species. The overall percentage of polymorphism (PP) among all species tested was 95%, with the mean genetic similarity (GS) was 0.34. Least PP (17.35%) and highest GS (0.60) was found between pairs of J. podagrica and J. multifida. The correlation between the PP with GS was relatively high (0.75). The farthest and closest genetic distance was found between J. curcas/J. gossypifolia and J. podagrica/J. multifida, respectively. Further, selected primers from this study can be utilized in species differentiation, molecular identification of interspecific hybrids, and exploiting the genetic resource.
Co-Authors ASEP SETIAWAN Bambang Heliyanto BAMBANG HELIYANTO NUR ASBANI SUDARSONO Sudarsono Sudarsono