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All Journal Biodiversitas Journal of Biological Diversity Nusantara Bioscience
Ratna Susandarini
Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

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Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia: - Ratna Susandarini; Siti Subandiyah; Budi Setiadi Daryono; Rugayah
Biodiversitas Journal of Biological Diversity Vol. 21 No. 6 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210608

Abstract

Abstract. Susandarini R, Subandiyah S, Daryono BS, Rugayah. 2020. Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia. Biodiversitas 21: 2390-2395. Citrus maxima (Burm.) Merr. (pomelo) as a minor fruit crop deserve attention on its phenotypic and genotypic variability to avoid the risk of extinction. Previous study showed that pomelo from Indonesia has high morphological variability, and thus it is interesting to explore its genotypic variability using molecular markers. Microsatellite is a molecular marker widely used in Citrus taxonomy studies. This study aimed at revealing microsatellite polymorphism and its potential application in cultivar characterization of C .maxima. Eighty accessions of C. maxima consisting of registered cultivars and landraces from Indonesia were used in this study. Analysis of microsatellite sequences from genomic DNA amplified using DY296883 primer showed that C. maxima microsatellite has high polymorphism in the form of repeat length variation of (GA)n, ranging from (GA)7 to (GA)19. This study proved the existence of high genotypic variability in C. maxima, and confirmed the role of microsatellite as a useful molecular marker for uncovering variability at intraspecific level. Observation of the microsatellite polymorphisms indicated that variability of (GA)n can be used to distinguish some pomelo cultivars.
Ethnobotanical study of plants used as food and for maternal health care by the Malays communities in Kampar Kiri Hulu, Riau, Indonesia Ratna Susandarini; Uswatun Khasanah; Nurma Rosalia
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220613

Abstract

Abstract. Susandarini R, Khasanah U, Rosalia N. 2021. Ethnobotanical study of plants used as food and for maternal health care by the Malays communities in Kampar Kiri Hulu, Riau, Indonesia. Biodiversitas 22: 3111-3120. Studies to reveal the diversity of food plants on communities living in remote areas with limited access to the market are important as an effort in documenting the traditional knowledge. The same applies to the diversity of medicinal plants used in maternal health care for communities that have limited access to public health facilities. The documentation of ethnobotanical knowledge is not only for the purpose of developing the potential of these plants, but also could have an impact on their conservation in nature. This study aims to document the traditional knowledge on the diversity of food plants and medicinal plants used in maternal health care by Malays tribes living within the Bukit Rimbang Bukit Baling Wildlife Reserve, Kampar Kiri Hulu Subdistrict, Kampar District, Riau Province, Indonesia. The research was conducted in three villages by collecting data through interviews and followed by fieldwork to collect plant specimens for identification. Data on food plant diversity data were obtained from 20 informants, while data on the diversity and use of medicinal plants for maternal health care were obtained from 73 informants. The results showed that there were 76 species of food plants from 35 families. These food plants were used as secondary food ingredients, vegetables, fruit and spices with most of these plants were obtained from the yard of the house. Plants used for maternal health care identified from this study were 34 species from 26 families. These plants were used for various purposes during pregnancy, child delivery, postpartum recovery, and infant health care. The diversity of food plants and medicinal plants for maternal health care documented in this study showed the valuable role of plant resources in supporting daily needs and health care of the communities living in the fringe of forest area.
Variation on morphology and spore characters of Dicranopteris and Sticherus (Gleicheniaceae) from Rokan Hulu District, Riau, Indonesia Afni Atika Marpaung; Ratna Susandarini
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221041

Abstract

Abstract. Marpaung AA, Susandarini R. 2021. Variation on morphology and spore characters of Dicranopteris and Sticherus (Gleicheniaceae) from Rokan Hulu District, Riau. Biodiversitas 22: 4475-4486. Dicranopteris and Sticherus are genera in the family Gleicheniaceae which have unique habits and distinctively specific growth with the presence of a bud on the forking branches. This study aims to reveal the species diversity of Gleicheniaceae members found in the forest area of Bukit Suligi Tandun, Rokan Hulu District, Riau and to document variations in their morphological characters and spores. Eleven collections were obtained from the exploration in the study area. The identification of plant specimens showed that these eleven taxa consisted of Dicranopteris curranii, Dicranopteris speciosa, seven varieties of Dicranopteris linearis, and two varieties of Sticherus truncatus. Seven varieties within D. linearis were D. linearis var. linearis, D. linearis var. alternans, D. linearis var. altissima, D. linearis var. demota, D. linearis var. inaequalis, D. linearis var. subspeciosa, and D. linearis var. tetraphylla. The only one species of Sticherus found was S. truncatus which consisted of two varieties, namely S. truncatus var. truncata and S. truncatus var. involuta. Morphological characters were observed from fertile specimens, while spore characters were observed from spore microscopic slides prepared using acetolysis method. There were 42 morphological characters and spores examined and used as the basis for determining the phenetic relationships using cluster analysis and principal component analysis. The results of cluster analysis showed the formation of two clusters that clearly separated the genus Sticherus from Dicranopteris. The three species of Dicranopteris were also clearly separated from each other in different sub-clusters. The result of principal component analysis indicated characters that contribute to the grouping of the eleven taxa and was able to explain the distinguishing characters in each group recognized from the cluster analysis. Overall, the results of this study contributed to documenting in detail the morphological variations in sporophyte and spore characters in eleven taxa of Dicranopetris and Sticherus, as well as proving the role of morphological and spore characters in the classification and defining phenetic relationships using numerical taxonomic approach.
Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia Lalu Zulkan Jayadi; Ratna Susandarini
Nusantara Bioscience Vol. 12 No. 2 (2020)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n120203

Abstract

Abstract. Jayadi LZ, Susandarini R. 2020. Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia. Nusantara Bioscience 12: 97-108. Honey is a natural product with a variety of benefits that commonly used as food sweeteners, health supplements, and traditional medicine. There has been no comprehensive publication regarding the diversity of pollen contained in honey produced by stingless bee Tetragonula laeviceps and Heterotrogona itama from Lombok. This study aimed to reveal the diversity of pollen content in honey through melissopalynological analysis of honey samples produced by two species of stingless bees T. laeviceps and H. itama in Lombok Island. This melissopalynological study was performed on honey samples obtained from nine villages from nine sub-districts representing the three districts on Lombok Island. Pollen extraction from honey was carried out using standard methods for melissopalynological analysis. The diversity of pollen recovered from honey varied from 15 to 41 pollen types. Total number of plant species whose pollen was recovered from honey samples was 127 which consisted of 61 families. The occurrence of predominant pollen type in particular unifloral honey samples indicated their botanical origin and presumed geographical origin of honey. Results of this study are useful in confirming botanical origin of honey and generating information on plants potential as food source for sustainable beekeeping in Lombok Island.
Co-Authors Afni Atika Marpaung Budi Setiadi Daryono Lalu Zulkan Jayadi Nurma Rosalia Rugayah Siti Subandiyah Uswatun Khasanah