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All Journal Biodiversitas Journal of Biological Diversity
Didik Wahyudi
Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik Ibrahim Malang. Jl. Gajayana No. 50, Malang 65144, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Genome evaluation of banana cultivars based on morphological character and Inter-Simple Sequence Repeat (ISSR) molecular marker Didik Wahyudi; Uslan; KHAFIDHOTUR RIFLIYAH
Biodiversitas Journal of Biological Diversity Vol. 21 No. 7 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210715

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Abstract. Wahyudi D, Rifliyah K, Uslan. 2020. Genome evaluation of banana cultivars based on morphological character and Inter-Simple Sequence Repeat (ISSR) molecular marker. Biodiversitas 21: 2982-2990. This study aims to evaluate the genome group and to investigate the genetic variability of banana cultivar using morphological character and ISSR molecular marker. Fourteen banana cultivars with genomic group AA, AAA, AAB, ABB, and BB was used. All samples were Identified morphologically based on minimal descriptors for bananas issued by the International Plant Genetic Resources Institute (IPGRI). Total genomic DNA was extracted using DNA Isolation Kit Promega Wizard®. ISSR primer was used in this study, including UBC834, UBC835, UBC843, UBC848, and UBC855. Clustering analysis was used to evaluate genome grouping of the banana cultivar. Clustering analysis of morphological character was performed using the unweighted pair group method with arithmetic mean (UPGMA) algorithm and Bray-Curtis coefficient similarity using Paleontological Statistics (PAST) software. Morphological and ISSR was successfully differentiate banana cultivars and relatively similar to previous genome grouping. However, pisang Triolin (AAB) must be evaluated since it belongs to AAA group. The identification of cultivars and classification of their genome groups based on morphological characteristics and proved by molecular markers will strengthen the establishment of Musa improvement strategy especially in Indonesia and ease the breeder to identify desirable traits of progenitor to be included in the breeding program.
Ethnobotanical study of medicinal plants used for maintaining stamina in Madura ethnic, East Java, Indonesia Akhmad Fathir; MOCH. HAIKAL; Didik Wahyudi
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220147

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Abstract. Fathir A, Haikal M, Wahyudi D. 2021. Ethnobotanical study of medicinal plants used for maintaining stamina in Madura ethnic, East Java, Indonesia. Biodiversitas 22: 386-392. The use of herbal medicine that expands rapidly across the world opens a lot of opportunities for drug exploration and discovery through ethnobotanical study. Therefore, countries with high level of biological and cultural diversity like Indonesia have great opportunities to achieve this goal, and even Indonesia has its own term for a group of herbal medicine, called jamu. This research aimed to conduct ethnobotanical study of jamu used by Madura ethnic in Pamekasan District, Madura Island, Indonesia by focusing on the specific use of it for maintaining stamina. Structured interviews with purposively selected respondents of 80 knowledgeable elder women were performed to gain insights about the medicinal herb used by them. Our study found 10 recipes of jamu used by Madura ethnic for maintaining stamina. These recipes contain 19 medicinal plants belonging to 16 genera and 11 families, with the family of Zingiberaceae contributed to the largest share of species used with eight species. Turmeric (Curcuma longa) was the species with the highest use-value by the respondents and the rhizomes were the most favorable plant organ used. In conclusion, the diversity of medicinal plants used by Madura ethnic could contribute to the development of new plant-based drugs, especially those for maintaining stamina.
Co-Authors Akhmad Fathir KHAFIDHOTUR RIFLIYAH MOCH. HAIKAL Uslan