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Nur Prihatiningsih
Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Suparno, Purwokerto Utara, Banyumas 53123, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Biodiversitas Journal of Biological Diversity Vol. 21 No. 9 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210934

Abstract

Abstract. Prihatiningsih N, Arwiyanto T, Hadisutrisno B, Widada J. 2020. Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum. Biodiversitas 21: 4199-4204. Ralstonia solanacearum is one of the important wilt pathogens that significantly decreased potato yields in Indonesia. One of the strategies to control wilt disease is the use of Bacillus sp. that isolated from potato rhizosphere. Bacillus sp. is an antagonist against some phytopathogenic fungi and bacteria.   The objectives of the study were to identify 5 strains of Bacillus spp. (B46, B209, B211, B298, and B315) that is capable of suppressing the growth of R. solanacearum. Experimental assays were performed to determine the growth inhibition of R. solanacearum by culturing in a two-layer medium. The identification of Bacillus spp. was performed based on physiological and biochemical characters and molecular identification. Results showed that all of Bacillus spp. are capable of suppressing the growth of R. solanacearum. B. subtilis B315 isolate was the most effective biocontrol agent in inhibiting R. solanacearum growth by a bacteriostatic mechanism.  All Bacillus spp.  have similarities in physiological and biochemical characters. Based on the molecular analysis, Bacillus sp. B46, B209, B211, and B298 were homologous to B. subtilis subspecies spizizeni RRLKE2, whereas Bacillus sp. B315 was homologous to B. subtilis strain WIFD5. 
Extracellular protease from Bacillus subtilis B315 with antagonistic activity against bacterial wilt pathogen (Ralstonia solanacearum) of chili : - Nur Prihatiningsih; ARI ASNANI; HERU ADI DJATMIKO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220327

Abstract

Abstract. Prihatiningsih N, Asnani A, Djatmiko HA. 2021. Extracellular protease from Bacillus subtilis B315 with antagonistic activity against bacterial wilt pathogen (Ralstonia solanacearum) of chili.  Biodiversitas 22: 1291-1295. Antagonistic bacteria isolated from a healthy potato are potential biocontrol agents against a number of bacterial pathogens. The capability of antagonistic bacteria to produce extracellular protease could be considered as an indicator of their antagonistic capacity. The objective of this research was to study the optimum activity of protease produced by Bacillus subtilis B315 to control Ralstonia solanacearum causing wilt of chili. In this research were optimum incubation time, pH and crude extract protease concentration of B. subtilis B315 examined for maximum production of extracellular protease. The results showed that B. subtilis B315 was capable of producing protease at optimum incubation time of 30 min, pH 9, and crude extract protease concentration 0.50% with an activity of 1.3185 U/mL. Protease-producing capability of B. subtilis B315 was shown by the formation of a clear zone around the colony of B. subtilis B315 grown on skim-milk agar medium. B. subtilis B315 was capable of inhibiting the growth of R. solanacearum in vitro as indicated by 32 mm clear zone. Furthermore, in chili application of B subtilis showed that this bacteria was capable of controlling chili bacterial wilt caused by R. solanacearum with effectiveness of 60.89%. The prospective of B. subtilis B315 could be formulated as biopesticide.
Co-Authors ARI ASNANI Bambang Hadisutrisno HERU ADI DJATMIKO Jaka Widada Triwidodo Arwiyanto