Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
AGUS HERY SUSANTO
Faculty of Biology, Universitas Jenderal Soedirman. Jl. dr. Suparno 63, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA) AGUS HERY SUSANTO; Murni Dwiati; Salabila Pratiwi
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211122

Abstract

Abstract. Susanto AH, Dwiati M, Pratiwi S. 2020. Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA). Biodiversitas 21: 5164-5169. Ogiera (Eleutheranthera ruderalis) and nodeweed (Synedrella nodiflora) are two different weed species belonging to the family Asteraceae commonly found in tropical regions. At a glance, both species show highly identical morphology, thus leading to difficulty in distinguishing between them. Therefore, molecular data based on particular markers are required. Here, we use an intergenic spacer (IGS) in the cpDNA, i.e., trnT(UGU)-trnL(UAA), as the molecular marker to reveal the difference between the two species. A pair of PCR universal primers, i.e., B48557 as the forward primer and A49291 as the reverse primer, were employed to amplify the marker. Sequence alignment was performed by the use of ClustalW implemented in Bioedit version 7.0.4.1. The results revealed that some differences with respect to both indel and base substitution were observed. Overall, this led to longer IGS trnT(UGU)-trnL(UAA) sequences of E. ruderalis than those of S. nodiflora. Although no direct relationship between the genetic and phenotypic dissimilarities was proven, coincidence seemed likely to exist. This provides molecular evidence that the two phenotypically similar species are genetically different from each other.
Shoot regeneration in Nepenthes mirabilis as affected by flurprimidol and GA3 application MURNI DWIATI; PUDJI WIDODO; AGUS HERY SUSANTO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 7 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240756

Abstract

Abstract. Dwiati M, Widodo P, Susanto AH. 2023. Shoot regeneration in Nepenthes mirabilis as affected by flurprimidol and GA3 application. Biodiversitas 24: 4168-4174. Nepenthes mirabilis (Lour.) Druce. is a pitcher plant species at a higher risk of extinction mainly due to overexploitation. Previous investigations have shown that in vitro micropropagation can be used as an approach for ex-situ conservation of N. mirabilis. Therefore, this study aimed to assess the new shoot formation of N. mirabilis under in vitro conditions stimulated with the application of flurprimidol and GA3. The three concentrations of flurprimidol and GA3 used as treatments were arranged factorially in a Randomized Complete Block Design (RCBD), with internodes 2, 3, 4, and 5 serving as blocks. Based on the results, F1G0 (flurprimidol of 1 mgL-1 without GA3) had the highest shoot formation, which was not accompanied by sufficiently high contents of chlorophyll, leading to improper shoot development. Despite the smaller number of newly formed shoots in F2G2 (flurprimidol of 2 mgL-1 and GA3 of 2 mgL-1), significantly higher chlorophyll contents were observed, enabling better development of shoot. Leaf length and shoot diameter also showed similar results under F2G2, indicating that higher concentrations of exogenous GA3 were necessary for better shoot development. This indicated that appropriate concentrations of both plant growth regulators should be applied for micropropagation of N. mirabilis in support of ex-situ conservation.
Co-Authors MURNI DWIATI Murni Dwiati PUDJI WIDODO Salabila Pratiwi