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All Journal Biodiversitas Journal of Biological Diversity Nusantara Bioscience
ESTRI LARAS ARUMINGTYAS
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65415, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

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Local snake fruit conservation in East Java, Indonesia: Community knowledge and appreciation Novita Indah; SERAFINAH INDRIYANI; ESTRI LARAS ARUMINGTYAS; RODIYATI AZRIANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220151

Abstract

Abstract. Indah NK, Indriyani S, Arumingtyas EL, Azrianingsih R. 2021. Local snake fruit conservation in East Java, Indonesia: Community knowledge and appreciation. Biodiversitas 22: 416-423. The community-based conservation of plant species diversity by variety of utility is an effective method for preserving local plants. Salak or the ‘snake fruit’ from East Java is an important fruit plant species in East Java. However, recent dramatic decline in its production and consumption occurred due to land-use change of plantation into resident or settlement areas. This study aims to analyze the value of local knowledge and appreciation and discusses the conservation strategy to cope with the situation. This descriptive research adopted 328 respondents from 10 local and introduced snake fruit cultivation areas in East Java. Respondents were divided into four age categories: young (17-30 years), adults (30-45 years), middle-aged (45-60 years), and old (>60 years). The questioner consisted of closed and open questions about knowledge (9 questions) and appreciation (13 questions). There were variations in the level of knowledge and appreciation between locations and ages. The highest appreciation about snake fruit utilization was shown by the middle-aged group (85.53% and 75% respectively) based on the age group. In comparison, the lowest scores were shown in the young group (79.82% and 59.80% respectively). The points that support appreciation such as benefits  and value of snake fruit for societal support. Based on the snake fruit cultivation area, the highest score for knowledge and appreciation of its value was found in Bojonegoro (98.1% and 87.01% respectively) while these were the lowest in Trenggalek (68.0% and 57.10% respectively). Appreciation supports community-based conservation through snake fruit festivals, product diversification i.e. making processed foods, and its use in religious ceremonies.
Molecular identification of Scopellaria marginata from East Java, Indonesia, based on trnL-UAA and trnL-trnF intergenic spacer regions TURHADI TURHADI; BRILIYAN NATALINA SUDARJAYANTI; FIFI MAR’ATUN SOLIHAH; RODIYATI AZRIANINGSIH; MUFIDAH AFIYANTI; ESTRI LARAS ARUMINGTYAS
Nusantara Bioscience Vol. 16 No. 1 (2024)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n160114

Abstract

Abstract. Turhadi, Sudarjayanti BN, Solihah FM, Azrianingsih R, Afiyanti M, Arumingtyas EL. 2024. Molecular identification of Scopellaria marginata from East Java, Indonesia, based on trnL-UAA and trnL-trnF intergenic spacer regions. Nusantara Bioscience 16: 111-118. Scopellaria marginata (Blume) W.J.de Wilde & Duyfjes is a wild species in Cucurbitaceae, which was recorded as new expanding their distribution in East Java, Indonesia. The trnL-UAA and trnL-trnF intergenic spacer (IGS) sequences of Scopellaria are still limited in publicly accessible databases. This study aimed to evaluate the trnL-UAA and trnL-trnF IGS sequences of S. marginata from Malang, East Java. Total DNA of S. marginata was used to amplify the trnL-UAA and trnL-trnF IGS region. Then, the PCR products are sequenced using bi-directional Sanger dideoxy sequencing to obtain the DNA sequence of those two regions. The results showed that the partial sequences of S. marginata for trnL-UAA ranged from 528 to 571 bp, while the sequence for trnF-trnL IGS ranged from 434 to 445 bp. The S. marginata samples are similar to S. marginata in the database with similarity levels of 97.14-97.50% and 98.36-98.61%, respectively, based on the trnL-UAA and trnL-trnF IGS. Both trnL-UAA and trnL-trnF IGS showed a Correct Assignment Rate (CAR) of 100% for S. marginata. Two-dimensional DNA barcoding with lengths 505 and 417 bp for trnL-UAA and trnL-trnF IGS proposed as specific barcodes for S. marginata. These results prove that Malang, East Java was an additional distribution area for S. marginata in Indonesia.
Co-Authors BRILIYAN NATALINA SUDARJAYANTI FIFI MAR’ATUN SOLIHAH MUFIDAH AFIYANTI Novita Indah RODIYATI AZRIANINGSIH SERAFINAH INDRIYANI TURHADI TURHADI