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All Journal Biodiversitas Journal of Biological Diversity
Yan Ramona
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana. Jl.Raya Kampus Unud No. 9, Jimbaran, Badung 80361, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens Yan Ramona; IDA BAGUS GEDE DARMAYASA; ANAK AGUNG NGURAH NARA KUSUMA; Martin Line
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220136

Abstract

Abstract. Ramona Y, Darmayasa IBG, Kusuma AANN, Line MA. 2021. Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens. Biodiversitas 22: 298-303. This study investigated the inhibitory potential of diversity of antagonist bacteria residing in the rhizosphere zone and mature compost to counter fungal plant pathogens. Soils collected from rhizosphere of lettuce farms in Bali-Indonesia and Tasmania-Australia, mature compost, commercial biocontrol (Dipel®), and laboratory contaminants with significant inhibition against tested fungal pathogens were used as sources of antagonist bacteria. These antagonists were isolated by applying dilution and spread method on trypticase soya agar (TSA) or potato dextrose agar (PDA), and their ability to inhibit Sclerotinia minor, Sclerotinia sclerotiorum, Fusarium spp., and Rhizoctonia solani was assessed in dual culture assays. The results showed that 67 out of more than 100 isolates had antagonistic activity in vitro against at least one of tested fungal pathogens. In the preliminary identification, Bacillus spp. or Pseudomonas spp. were found to be pre-dominant isolates. Following screening studies in a non-replicated glasshouse experiment against S. minor and S. sclerotiorum, 8 of the most promising isolates were further identified using molecular methods based on their 16s rDNA sequences aligned with those deposited at the GeneBank. These 8 isolates were identified as Pseudomonas corrugata, Bacillus megaterium, Bacillus polymyxa, Bacillus mojavensis, Bacillus pumilus, Bacillus thuringiensis, Exiguobacterium acetylicum, and Chryseobacterium indologenes.
Biological control of Sclerotinia minor attack on pyrethrum plants by Trichoderma harzianum in glasshouse experiment Yan Ramona; Ida Bagus Gede Darmayasa; Martin A Line
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230655

Abstract

Abstract. Ramona Y, Darmayasa IBG, Line MA. 2022. Biological control of Sclerotinia minor attack on pyrethrum plants by Trichoderma harzianum in glasshouse experiment. Biodiversitas 23: 3264-3269. The aim of this research was to elucidate the efficacy of Trichoderma harzianum (isolate Td22) grown in a ratio of 2:8 millet seeds and wood fiber waste (WFW) compost mixture to suppress Sclerotinia minor infection in pot trails on the pyrethrum plants (in 0.5 L pots). The pots were filled with soil and mixed with Td22-grown WFW compost to obtain a concentration of 5% v/v. The S. minor (fungal pathogen) previously grown in millet seeds amounted at 2.0 g per pot, was then evenly inoculated at 2 cm below the surface of potting mix. Soil without compost amendment, amended with pathogen only, or without pathogen inoculation served as controls. All pots were acclimatized for 4 days in a shade house prior to transplanting (4 seedlings per pot) of pyrethrum seedlings (aged of 3 weeks). Eight replications per treatment were run for 8 weeks. The results showed that 5% v/v compost-grown Td22 provided 78% protection to pyrethrum plants at week 8. Each surviving plant in Td22-treated pots also showed significantly higher average dry weight (p<0.05) than those planted in S. minor control treatment, indicating that Td22 has a potential to be developed as a novel fungal antagonist or a plant growth-promoting fungus.
Co-Authors ANAK AGUNG NGURAH NARA KUSUMA IDA BAGUS GEDE DARMAYASA Martin A Line Martin Line