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All Journal Biodiversitas Journal of Biological Diversity
MUFASIRIN MUFASIRIN
Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Mulyorejo, Kampus C Unair, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Surface ultrastructure of Blastocystis sp. isolated from cattle NI KOMANG APRILINA WIDISUPUTRI; NUNUK DYAH RETNO LASTUTI; ENDANG SUPRIHATI; POEDJI HASTUTIEK; HANI PLUMERIASTUTI; MUFASIRIN MUFASIRIN; HENI PUSPITASARI; Lucia Tri Suwanti
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220356

Abstract

Abstract. Widisuputri NKA, Lastuti NDR, Suprihati E, Hastutiek P, Plumeriastuti H, Mufasirin, Puspitasari H, Suwanti LT. 2021. Surface ultrastructure of Blastocystis sp. isolated from cattle. Biodiversitas 22: 1514-1518. Blastocystis sp is a protozoan parasite commonly detected in the intestinal tract of humans and various animals that causes zoonotic blastocystosis. The pathogenic potential of Blastocystis is still being evaluated, some Blastocystis sp are completely pathogenic, while others can be considered commensal and hypothetical, related to the role of the surface coat of Blastocystis sp. This study aimed to compare the surface ultrastructure of Blastocystis sp. in cattle with diarrhea and non diarrhea by Scanning Electron Microscope (SEM). Four Blastocystis sp. isolates were selected from the faeces of four positives cattle which consisted of two diarrhea and two non-diarrhea cattle. The result showed that Blastocystis sp. in cattle appeared in round shape and reproduced by binary fission.  The surface cell of Blastocystis sp. isolates from diarrhea cattle had a rough surface while organism of non diarrhea cattle isolates was very smooth. Bacteria were seen attached to the surface of Blastocystis sp. from diarrhea cattle faeces. In conclusion, the features of the surface structure of Blastocystis sp. correlated with symptomatic appearance. The surface structure of Blastocystis sp. isolates from cattle with diarrhea was rougher than non diarrhea.
The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro ZHAZA AFILILLA; Lucia Tri Suwanti Suwanti; MUFASIRIN MUFASIRIN; HENI PNY KOERNIAWAN; MOCHAMAD DONNY KOERNIAWAN; EKO AGUS SUYONO; ARIEF BUDIMAN; ULFAH JUNIARTI SIREGAR
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231028

Abstract

Abstract. Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some microalgae have many bioactivities, such as immunomodulatory activity. This study aimed to determine Glagah consortium microalgae polysaccharide (GCMP) and polysaccharide of Spirulina platensis (PSP) as an immunomodulator based on its effect on splenocyte proliferation and cytokine production in vitro. Crude polysaccharides were extracted by lye extraction method. Splenocytes were obtained from spleen of healthy mice and cultured in RPMI 1640 medium with and without Concanavalin A (Con A). Cells (3×105 per well) were treated with polysaccharide with dose gradually 0, 1.25, 2.5, 10 and 20 µg/mL. Splenocytes proliferation were observed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The supernatant was taken for the detection of cytokines using enzyme-linked immunoassay (ELISA). The results showed that GCMP, both with and without Con A at a concentration of less than 10 mg/mL decreased cell viability, on the other hand, a concentration of 10-20 mg/mL had a proliferative effect. Splenocytes were shown a proliferative effect at all concentrations of PSP, both with and without Con A. The concentration of polysaccharides, both GCMP and PSP, did not affect cytokine levels. In conclusion, based on the proliferation of splenocytes, GCMP has an immunomodulatory effect depending on its concentration.
Co-Authors ARIEF BUDIMAN EKO AGUS SUYONO ENDANG SUPRIHATI HANI PLUMERIASTUTI HENI PUSPITASARI HENI PNY KOERNIAWAN Lucia Tri Suwanti Lucia Tri Suwanti Suwanti NI KOMANG APRILINA WIDISUPUTRI NUNUK DYAH RETNO LASTUTI POEDJI HASTUTIEK ULFAH JUNIARTI SIREGAR ZHAZA AFILILLA