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All Journal Biodiversitas Journal of Biological Diversity
MOCH. AFFANDI
Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Unair, Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, East Java, Indonesia FATIMAH FATIMAH; FATICHATUS SUROIYAH; NIKMATUS SOLIKHA; NAZIL DWI RAHAYUNINGTYAS; TINI SURTININGSIH; TRI NURHARIYATI; NI’MATUZAHROH NI’MATUZAHROH; MOCH. AFFANDI; ALMANDO GERALDI; AHMAD THONTOWI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230622

Abstract

Abstract. Fatimah, Suroiyah F, Solikha N, Rahayuningtyas ND, Surtiningsih T, Nurhariyati T, Ni’matuzahroh, Affandi M, Geraldi A, Thontowi A. 2022. Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia. Biodiversitas 23: 2957-2965. This study aims to isolate, identify, and investigate the antimicrobial activity of actinomycetes isolated from mangrove soil of the Mangrove Center Tuban in Jenu Village, Tuban District, Indonesia. Soil sampling was carried out with a purposive sampling technique. The dry-heating and pour plate methods were used to isolate actinomycetes. Four isolates (LIB.2; LIA.4; LIA.9; and LIA.10) were selected and tested for their antimicrobial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 using disc diffusion method. Colony morphology characterization and Gram staining were then conducted on the 4 isolates. The identification of LIB.2 was performed by biochemical assay and 16S rRNA gene sequence analysis. The results showed that crude antimicrobial extracts (CACs) of all isolates inhibited the growth of E. coli but not S. aureus and C. albicans. Isolate LIB.2 showed the highest inhibition zone with a diameter of 15,65 ± 2,33 mm, followed by LIA.9 (10,19 ± 2,78 mm), LIA.4 (9,80 ± 0,47), and LIA.10 (8,75 ± 0,42 mm). The most potential isolate was identified as a member of Streptomyces genera. Based on biochemical assay and 16S rRNA gene sequence analysis, LIB.2 was identified as Streptomyces vellosus strain LIB.2.
Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture NI’MATUZAHROH NI’MATUZAHROH; MOCH. AFFANDI; AGUS SUPRIYANTO; BERLIAN RUSTANTINA; LAILY AINUN JAIYAH; AISYAH RAHMAWATI; HESTI NURHAYATI; SILVIA KURNIA SARI; ANA MARIATUL KHIFTIYAH; DAMAN HURI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250146

Abstract

Abstract. Ni’matuzahroh, Affandi M, Supriyanto A, Rustantina B, Jaiyah LA, Rahmawati A, Nurhayati H, Sari SK, Khiftiyah AM, Huri D. 2024. Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture. Biodiversitas 25: 392-403. Biofertilizer is an alternative to chemical fertilizer that can enhance plant growth but is specific to certain plants and soils. Various biofertilizers have been produced, but the specific biofertilizers to enhance durian growth have not been widely available. This study aims to determine the potential of hydrolytic enzyme-producing bacteria, one of the microbial components of biofertilizers, in the De Durian Park (DDP) area, Wonosalam, Jombang District, Indonesia. The bacteria were isolated using the selective medium plating technique. The obtained isolates were screened for their ability to produce cellulase, amylase, protease, and lipase. The clear zone around the colony indicated the ability of the isolates to produce hydrolytic enzymes, and then the clear zone index was obtained. The best three isolates for each enzyme production were characterized macroscopically, microscopically, and biochemically, then identified using 16S rRNA gene sequence analysis. Fifty-two isolates were successfully obtained from the soil of DDP. The best three cellulolytic bacteria had indices ranging from 1.83 to 2.14, categorized as moderate-strong, identified as Bacillus anthracis, B. pacificus, and B. cereus. The amylolytic bacteria ranged from 1.25 to 1.35, categorized as moderate, and identified as Bacillus anthracis, B. paranthracis, and B. paramycoides. The proteolytic bacteria ranged from 1.33 to 1.50, categorized as moderate, identified as B. pumilus, B. paramycoides, and B. cereus. The lipolytic bacteria ranged from 1.94 to 3.87, categorized as moderate-strong, and were identified as Enterobacter sichuanensis, B. pacificus, and E. asburiae. These cellulolytic, amylolytic, proteolytic, and lipolytic bacteria have the potency as candidates for a biofertilizer formula.
Co-Authors AGUS SUPRIYANTO AHMAD THONTOWI AISYAH RAHMAWATI ALMANDO GERALDI ANA MARIATUL KHIFTIYAH BERLIAN RUSTANTINA DAMAN HURI FATICHATUS SUROIYAH FATIMAH FATIMAH HESTI NURHAYATI LAILY AINUN JAIYAH NAZIL DWI RAHAYUNINGTYAS NIKMATUS SOLIKHA NI’MATUZAHROH NI’MATUZAHROH SILVIA KURNIA SARI TINI SURTININGSIH TRI NURHARIYATI