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All Journal Biodiversitas Journal of Biological Diversity
ANTON MUHIBUDDIN
Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Screening of endophytic fungi as potential antagonistic agents of Pyricularia oryzae and evaluation of their ability in producing hydrolytic enzymes NOVIA DWI PUTRI; LILIEK SULISTYOWATI; LUQMAN QURATA AINI; ANTON MUHIBUDDIN; IRISA TRIANTI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230248

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Abstract. Putri ND, Sulistyowati L, Aini LQ, Muhibuddin A, Trianti I. 2022. Screening of endophytic fungi as potential antagonistic agents of Pyricularia oryzae and evaluation of their ability in producing hydrolytic enzymes. Biodiversitas 23: 1048-1057. One of the most important phytopathogenic fungi is Pyricularia oryzae, as it is the causative agent of rice blast diseases, which is the most destructive and detrimental disease in rice. Biological control using endophytic fungi can be an alternative to control blast diseases. Endophytic fungi are fungi that colonize internal plant tissues without apparently damaging the host. The aim of this study was to isolate endophytic fungi that produce hydrolytic enzymes and to investigate their ability to suppress blast disease. In this study, fifty strains of endophytic fungi were successfully obtained from rice leaves. Three fungi with the highest inhibition against P. oryzae were identified as Trichoderma asperellum, Curvularia chiangmaiensis, and Fusarium solani by analyzing fungal ITS sequence. They can produce chitinase ranging from 1.43 to 151 ?g/mL and cellulase ranging from 1.83 to 4.09 ?g/mL, which were hydrolytic enzymes that account for the lysis of phytopathogens. This enzymatic activity can cause damage and degradation of cell walls. SEM results revealed that these endophytic fungi probably excreted wall lytic enzymes or antifungal substances, inflicting wrinkles and disintegrating P. oryzae mycelium.
Screening of yeast isolates for the biocontrol of Sclerotium rolfsii NURHALIMAH NURHALIMAH; ANTON MUHIBUDDIN; MUHAMMAD AKHID SYIB’LI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230759

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Abstract. Nurhalimah, Muhibuddin A, Syib’li MA. 2022. Screening of yeast isolates for the biocontrol of Sclerotium rolfsii. Biodiversitas 23: 3820-3826. Sclerotium rolfsii is a fungal pathogen that can reduce peanut production. Biological control with yeast antagonist offers an environment friendly control for this pathogen. The aim of this study was to isolate and identify yeast antagonists, and to study their antagonistic mechanism and potential to control the peanut disease caused by S. rolfsii. The study was conducted from October 2020 to May 2021. The study consisted of four stages: isolation and identification of yeasts, yeasts antagonist testing against S. rolfsii, molecular identification of yeast NH4, and scanning electron microscope observation of NH4 against S. rolfsii. Based on the research, four different yeast isolates were isolated, identified, and found able to be developed as a biological control agent to inhibit the growth of S. rolfsii. Identification showed that NH1 was Saccharomyces sp. with inhibitory percentage of 9,26%, NH2 was Candida sp. with 22,21% inhibition of, NH3 Pichia sp. with 11,11% inhibition of, and NH4 was Wickerhamomyces sp. with an inhibitory percentage of 32,96%. The results of the yeast antagonism mechanism showed that NH1 (Saccharomyces sp.) and NH3 (Pichia sp) had not showed any mechanism while, NH2 (Candida sp.) had a competition and NH4 (Wickerhamomyces sp.) had an antibiosis mechanism. NH4 can suppress the growth of S. rolfsii by more than 30%, which has been identified as species of Wickerhamomyces sp. (NH4) with 99.84% homology with Wickerhamomyces anomalus CBS 5759 with accession number MH545921.1.
Co-Authors IRISA TRIANTI LILIEK SULISTYOWATI LUQMAN QURATA AINI MUHAMMAD AKHID SYIB’LI NOVIA DWI PUTRI NURHALIMAH NURHALIMAH