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All Journal Biodiversitas Journal of Biological Diversity
REINHARD PINONTOAN
Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang, Banten 15811, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Whole-genome analysis of Bacillus subtilis G8 isolated from natto DIKSON DIKSON; HANS VICTOR; DELVIN JONG; ASTIA SANJAYA; ARIELA SAMANTHA; JUANDY JO; REINHARD PINONTOAN
Biodiversitas Journal of Biological Diversity Vol. 23 No. 3 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230313

Abstract

Abstract. Dikson, Victor H, Jong D, Sanjaya A, Samantha A, Jo J, Pinontoan R. 2022. Whole-genome analysis of Bacillus subtilis G8 isolated from natto. Biodiversitas 23: 1293-1300. Bacillus subtilis-fermented soy-based food is associated with multiple health benefits. Various bacterial strains have been isolated from it, includingB. subtilisG8, recent isolation from Japanese natto commercially available in Indonesia. Both 16S rRNA gene sequencing and fibrinolytic activity characterization have been performed and published in prior studies. After comparison to the genome of a natto-isolated reference strain (i.e., B. subtilisBEST195), the B. subtilis G8 genome showed a similar guanine-cytosine (GC) content, predicted number of coding sequences (CDS) and predicted number of tRNA genes, but had a shorter sequence length and fewer predicted rRNA genes. Further analysis using multiple genome alignment with Mauve, average nucleotide identity (ANI) matrix calculation, and phylogenetic inference indicated that B. subtilis G8 was more related to natto-derived B. subtilis than to cheonggukjang-derived B. subtilis and B. subtilis 168. Finally, sequence analyses of a gene encoding nattokinase as well as two genes regulating poly-gamma-glutamic acid (?-PGA) production in B. subtilis G8, B. subtilis BEST195 and B. subtilis 168 clearly indicated that B. subtilis G8 is able to produce nattokinase and ?-PGA, which both contribute to natto’s fermentation process. Therefore, it is proposed that B. subtilis G8 should be reclassified as B. subtilis subsp. natto G8 to reflect that it is a natto-derived B. subtilis strain.
Trypan blue dye decolorization by Aeromonas caviae isolated from water sewage in Jakarta, Indonesia REINHARD PINONTOAN; TAN STEVEN RYAN SUSANTO; JAP LUCY; CHRISTINE ANGELINA; SAMUEL EMMANUEL SOENTORO; JONATHAN SUCIONO PURNOMO; MELANIE CORNELIA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 4 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250432

Abstract

Abstract. Pinontoan R, Susanto TSR, Lucy J, Angelina C, Soentoro SE, Purnomo JS, Cornelia M. 2024. Trypan blue dye decolorization by Aeromonas caviae isolated from water sewage in Jakarta, Indonesia. Biodiversitas 25: 1631-1637. The textile industry generates a substantial amount of hazardous chemical waste, which requires proper treatment to mitigate negative environmental and health consequences if left untreated. Studies on the removal of Trypan Blue (TB), a widely used commercial diazo textile dye, by indigenous bacteria are limited. Therefore, this study aimed to isolate, identify, and characterize microorganisms capable of decolorizing TB in textile dye-contaminated wastewater from Jakarta. Microorganisms were initially screened for decolorization activity in solid media containing TB. Among the isolated strains, TB2 isolate exhibited the highest decolorizing potential and was selected for further analysis. The morphological, biochemical, molecular, and phylogenetic characteristics of the TB2 isolate revealed that it belonged to the species Aeromonas caviae. To examine the ability of the isolate to remove TB, various culture conditions, such as pH, temperature, and agitation, were tested. The results demonstrated that the A. caviae TB2 strain could reduce up to 77.10% of TB (0.0025% (w/v)) under static conditions, pH 7.0, and 27°C over six days. To our knowledge, this is the first study to report the ability of the Aeromonas genus to decolorize TB. These results imply the potential use of A. caviae for decolorizing dye-bearing industrial wastewater.
Co-Authors ARIELA SAMANTHA ASTIA SANJAYA CHRISTINE ANGELINA DELVIN JONG DIKSON DIKSON HANS VICTOR JAP LUCY JONATHAN SUCIONO PURNOMO JUANDY JO MELANIE CORNELIA SAMUEL EMMANUEL SOENTORO TAN STEVEN RYAN SUSANTO