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All Journal Biodiversitas Journal of Biological Diversity
ANJA MERYANDINI
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Agatis, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Coupling Indonesian indigenous Citrobacter freundii and Chlorella pyrenoidosa strain on the anode of microbial fuel cell with various substrates IRFAN ANWAR FAUZAN; ANJA MERYANDINI; RONI RIDWAN; RUSLI FIDRIYANTO; NI WAYAN SRI AGUSTINI; DWI ANDREAS SANTOSA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230527

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Abstract. Fauzan IA, Meryandini A, Ridwan R, Fidriyanto R, Agustini NWS, Santosa DA. 2022. Coupling Indonesian indigenous Citrobacter freundii and Chlorella pyrenoidosa strain on the anode of microbial fuel cell with various substrates. Biodiversitas 23: 2471-2481. Microorganism plays a crucial role in the development of MFC systems. Indigenous to Indonesia, Citrobacter freundii GBH253 is a potential exoelectrogenic bacterium that could be developed into an MFC system. Coupling C. freundii GBH253 with potentially electricity-producing microalgae indigenous to Indonesia, such as Chlorella pyrenoidosa INK, in the anode of an MFC, could result in a more stable and higher electricity output. This study used C. freundii GBH253 and C. pyrenoidosa INK to produce electricity in various substrates. This research was conducted using a Factorial Randomized Block Design and Tukey’s test to determine significant differences between treatments. The result shows that electricity was generated in all treatments. The Bacterium-microalgae combination in acetate substrate can generate power density up to 211,97 mW m-2 and is the most stable compared to others. Bacterium dominates the electricity production in this combination, but the microalgae also play a role in producing electricity and increasing Chemical Oxygen Demand. The pH value of all treatments was higher than 7. Volatile Fatty Acids, like acetate and phenol, were produced in all treatments, whereas butyric acid and propionic acid were produced in several treatments. The Pearson correlation showed that some VFAs are highly correlated with power density.
Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads ANGGRAINI PUTRI UTAMI; FAHRURROZI FAHRURROZI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230813

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Abstract. Utami AP, Fahrurrozi, Meryandini A. 2022. Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads. Biodiversitas 23: 3960-3966. Pectinase is one of the enzymes often used in the industrial sector, especially in the food industry, such as extracting and clarifying juices. Previous studies have obtained pectinolytic microbial cultures of Bacillus sp. 2P11 isolated from cocoa beans. Pectinase production using pure pectin substrates such as citrus pectin or pectin from apples costs a lot because these pectins are expensive. Liquid substrate from cocoa pods TSH 858 and ICS 60 with a concentration of 10% is a good amount for pectinase production, and the percentage of total pectin from cocoa pods is 0.823%. The amount of activity of crude extract of pectinase enzyme is 416,780 mU/mL. Precipitation with ammonium sulfate and dialysis increased pectinase activity by 452,335 mU/mL and 586.88 mU/mL, respectively. Enzymes can be used repeatedly with enzyme immobilization. In this study, the immobilization of the enzyme entrapment method with sodium alginate and CaCl2 was designed with the response surface method. The optimum concentration of sodium alginate was 0.5% and 0.3 M CaCl2, with the percentage of an immobilized enzyme at 40.782%. Stability and repeated use of immobilized pectinase can be done up to 5 times.
Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics SINTA AGUSTINA; KOMANG GEDE WIRYAWAN; SRI SUHARTI; ANJA MERYANDINI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231243

Abstract

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.
Co-Authors ANGGRAINI PUTRI UTAMI DWI ANDREAS SANTOSA FAHRURROZI FAHRURROZI IRFAN ANWAR FAUZAN KOMANG GEDE WIRYAWAN NI WAYAN SRI AGUSTINI RONI RIDWAN RUSLI FIDRIYANTO SINTA AGUSTINA SRI SUHARTI