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All Journal Biodiversitas Journal of Biological Diversity
CHANATE WANNA
Department of Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University. U-Thong nok Rd., Dusit, Bangkok 10300, Thailand
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Detection of Staphylococcus aureus from contact surfaces of public buses in Bangkok and metropolitan area, Thailand NARUMON BOONMAN; JARUWAN CHUTRTONG; CHANATE WANNA; SIRIPHAN BOONSILP; SUPATRA CHUNCHOB
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230711

Abstract

Abstract. Boonman N, Chutrtong J, Wanna C, Boonsilp S, Chunchob S. 2022. Detection of Staphylococcus aureus from contact surfaces of public buses in Bangkok and metropolitan area, Thailand. Biodiversitas 23: 3395-3400. The purpose of present investigation was to determine the prevalence of different species of genus Staphylococcus on the contact surfaces of public buses in Bangkok and metropolitan area. A total of 180 samples were collected from handrails, seats, and window frames of each bus of 2 bus terminals, Bangkhen and Morchit, divided into 15 non-air-conditioned buses and 15 air-conditioned buses from each terminal. The samples were cultivated and identified by Gram’s stain and biochemical tests. The results showed that 76.7% from Bangkhen and 80% from Mochit were contaminated with Staphylococcus spp. Based on the type of vehicles, Staphylococcus spp. was found in 93.3% of air-conditioned buses and 63.3% of non-air-conditioned buses. Considering the sampling location, Staphylococcus spp. was found in 28.3% of handrails, 50% of seats and 51.7% of window frames. Four isolates of Staphylococcus aureus were discovered among 294 isolates in a total of Staphylococcus spp., including 3 isolates from Bangkhen and 1 isolate from Mochit. Only one isolate was contaminated on the non-air-conditioned bus, whereas 3 isolates were on the air-conditioned buses. Two isolates of them were found from seats and 2 isolates from window frames. Disk diffusion susceptibility test showed that 3 isolates of S. aureus resisted fusidic acid and fosfomycin. The results suggested that Staphylococcus spp. and S. aureus were prevalence in public transport systems which were a source of infection to the persons. Therefore, effectively controlling their distribution is necessary to reduce the risk of bacterial infections in public bus users.
Genotyping and physiological characteristics of Acanthamoeba isolated from beaches in Phuket Province, Thailand NARUMON BOONMAN; CHANATE WANNA; JARUWAN CHUTRTONG; SRILERT CHOTPANTARAT; SIRIPHAN BOONSILP
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230902

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Abstract. Boonman N, Wanna C, Chutrtong J, Chotpantarat S, Boonsilp S. 2022. Genotyping and physiological characteristics of Acanthamoeba isolated from beaches in Phuket Province, Thailand. Biodiversitas 23: 4377-4384. Acanthamoeba is widely distributed in water, soil, dust and air. Beaches are popular tourist destinations for water-related activities while they can also be reservoirs for Acanthamoeba to humans. This study aimed to determine the genotyping of Acanthamoeba in the beach environment and characterized their pathogenicity potential. A total of 63 samples were collected from six beaches in Phuket Province, Thailand. Twenty-one samples of each source including wet sand, dry sand and seawater, were collected and cultivated on 2% non-nutrient agar coated with heat-killed Escherichia coli. Acanthamoeba was found in 100% (n=21) wet sand, 52.4% (n=11) dry sand and 14.3% (n=3) seawater. Sequence analysis of the GTSA.B1 region in 18S rRNA revealed that 83.9% of sequenced isolates belonged to the T5 genotype and 16.1% belonged to the T4 genotype. The T5 genotype was predominant, differing from other reports of Acanthamoeba prevalence in natural sources. In vitro thermal and salt-tolerance showed that all isolates had growth rates similar to clinical Acanthamoeba isolates. This research was undertaken to increase awareness of human eye and brain infections caused by Acanthamoeba on beaches. Further studies on these Acanthamoeba isolates are still required such as co-culture assay between the trophozoites and human cell lines to confirm their pathogenicity as well as their susceptibility to the chemotherapeutic agents.
Local herbs for pain relief in the area of Tumbon Khao Hin Son, Chachoengsao, Thailand VACHIRAPORN PIKULTHONG; SUPAMAS BHIROMPAN; MANUSSAWEE DECHKLA; KANLAYA MOKKAPAN; NARUMON BOONMAN; CHANATE WANNA; KWANCHAYANAWISH MACHANA; SIRIRAT PHAKPAKNAM
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231007

Abstract

Abstract. Pikulthong V, Bhirompan S, Dechkla M, Mokkapan K, Boonman N, Wanna C, Machana K, Phakpaknam S. 2022. Local herbs for pain relief in the area of Tumbon Khao Hin Son, Chachoengsao, Thailand. Biodiversitas 23: 5012-5019. Local herbs have been used in Thailand for years. However, data collection on the use of local herbs in particular areas has not been thorough. This study aimed to survey local herbs treating pain relief in the area of Tumbon Khao Hin Son, Amphoe Phanom Sarakham, Chachoengsao, Thailand. Semi-structured interviews were used. Purposive sampling was used to determine the sample group. In the survey and study on botanical characteristics, from November 2019 - March 2020, 68 plants were identified and classified into 29 families. The local herbs which were mostly used for pain relief included 7 species from the Acanthaceae family, 6 types from Fabaceae, Lamiaceae, and 5 types from Zingiberaceae. The leaves of herbaceous plants had the highest frequency of use. A remedy, Ko-Klan, was found and prepared using a boiling method that could relieve pain. The surveyed herbs were used to treat 4 main symptoms which were body pain, stomachache, headache, and toothache. It was found that 36 species of herbs, or a majority of them, were used to directly relieve the pain. The interviews showed that 27% of the respondents had some home-grown herbs which were used for cooking and basic illness treatment. The age of the respondents was relevant to the use of herbs. The findings in this study will be the foundation for local herb studies which support and are aware of Thai local wisdom and knowledge as guidance to alter herbs for pharmaceutical purposes in the future.
Antimicrobial activities of endophytic bacteria isolated from Ageratum conyzoides Linn. NARUMON BOONMAN; JARUWAN CHUTRTONG; CHANATE WANNA; SIRIPHAN BOONSILP; SUPATRA CHUNCHOB
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240405

Abstract

Abstract. Boonman N, Chutrtong J, Wanna C, Boonsilp S, Chunchob S. 2023. Antimicrobial activities of endophytic bacteria isolated from Ageratum conyzoides Linn. Biodiversitas 24: 1971-1979. Endophytic bacteria were isolated from various parts of Ageratum conyzoides Linn. Total 35 isolates were obtained which were consisting of eight isolates from the roots (AconR1-AconR8), nine isolates from the stems (AconS1-AconS9), seven isolates from the leaves (AconL1-AconL7) and 11 isolates from the flowers (AconF1-AconF11). These endophytic bacteria were examined for their antimicrobial activities against human pathogenic bacteria and fungi. The AconR2 and AconR4 inhibited the growth of Shigella flexneri and Salmonella enterica ser. typhi, whereas Escherichia coli was only inhibited by AconR2. For the antifungal activities assay, all isolated endophytic bacteria revealed no effect on Candida albicans, while six endophytic isolates exhibited more than 80% mycelial growth inhibition against Microsporum canis. These highly effective isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The Bruker scores revealed that AconR2 and AconR4 were identified as Pseudomonas aeruginosa, AconS1 and AconS6 were classified as Enterobacter asburiae, Acon L5 and Acon F9 were identified as Bacillus cereus. The results indicated that these endophytic bacteria isolated from A. conyzoides effectively inhibited the growth of human pathogenic filamentous fungi and showed promise for further development as novel antifungal agents.
Co-Authors JARUWAN CHUTRTONG JARUWAN CHUTRTONG KANLAYA MOKKAPAN KWANCHAYANAWISH MACHANA MANUSSAWEE DECHKLA NARUMON BOONMAN SIRIPHAN BOONSILP SIRIPHAN BOONSILP SIRIRAT PHAKPAKNAM SRILERT CHOTPANTARAT SUPAMAS BHIROMPAN SUPATRA CHUNCHOB SUPATRA CHUNCHOB VACHIRAPORN PIKULTHONG