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All Journal Biodiversitas Journal of Biological Diversity
MORGAN OHIWAL
Faculty of Fisheries and Forestry, Universitas Muhammadiyah Maluku. Jl. KH. Ahmad Dahlan, Ambon 97128, Maluku, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Endophytic bacteria associated with Myristica fragrans Houtt: Improved media, bacterial population, preliminary characterization, and potential as antibacterials MELDA YUNITA; MORGAN OHIWAL; CHRISLAVENA SHINTA DIRKS; ONY WIBRIYONO ANGKEJAYA; NOOR ANDRYAN ILSAN; SUKMAWATI SUKMAWATI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230824

Abstract

Abstract. Yunita M, Ohiwal M, Dirks CS, Angkejaya OW, Ilsan NA, Sukmawati S. 2022. Endophytic bacteria associated with Myristica fragrans Houtt: Improved media, bacterial population, preliminary characterization, and potential as antibacterials. Biodiversitas 23: 4047-4054. We have investigated a similar study previously and only obtained 4 isolates that were able to inhibit pathogenic bacteria with a very small inhibition index (1.5 mm-3.4 mm) in NA media. Therefore, the current study was conducted by improving the NA media with the addition of 1% peptone and Myristica fragrans filtrate. The study aimed to evaluate the potential of endophytic bacteria as antibacterials in the modified NA media. Endophytic bacteria were isolated from 5 organs of M. fragrans and were grown on Nutrient Agar added with 1% peptone and M. fragrans filtrate. The total bacterial population was analyzed by the TPC method. Preliminary characterization consisted of macroscopic and microscopic observations. Antibacterial test was carried out by agar diffusion method. The total population of endophytic bacteria varied for all organs of M. fragrans, with the highest population was found in the seeds (1x105 CFU/gr), while the least was found in the pulp (9x103 CFU/gr). A total of 10 isolates were selected and preliminary characterization showed that endophytic bacteria had different macroscopic and microscopic characteristics. All isolates were able to inhibit the growth of Escherichia coli ATCC-27853 and Staphylococcus aureus ATCC-29213 with the largest inhibition zone index was obtained by isolate BJ1 (22.5 mm and 23.8 mm), while the smallest was obtained by isolate TD2 (12.5 mm and 13.6 mm) which were still categorized as strong inhibition. The study concluded that the addition of 1% peptone and M. fragrans filtrate in NA media was able to show far better results compared to our previous study and the strong antibacterials can be developed and formulated in the future.
Antibacterial activity of Paederia foetida leaves using two different extraction procedures against pathogenic bacteria MELDA YUNITA; MORGAN OHIWAL; MUHAMMAD ZEIN ELFITRASYA; HALIDAH RAHAWARIN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 11 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241110

Abstract

Abstract. Yunita M, Ohiwal M, Elfitrasya MZ, Rahawarin H. 2023. Antibacterial activity of Paederia foetida leaves using two different extraction procedures against pathogenic bacteria. Biodiversitas 24: 5920-5927. The objective of this study was to compare the effectiveness of the extraction methods between maceration and infundation of Paederia foetida (Linn.) leaves in suppressing the growth of Staphylococcus aureus and Escherichia coli with concentrations of 10, 25, 50, 75, and 100% using the disc-diffusion method. While phytochemical testing was performed by decoction method. The results of this study revealed that the macerated extract was able to inhibit both S. aureus and E. coli at all concentrations with the inhibition zones varied ranging from 5.8 ± 0.85 - 20.43 ± 0.06 mm and 4.9 ± 0.57 - 18.18 ± 0.67 mm, respectively. While the leaf infusion obtained from infundation method was found to be less able to inhibit both pathogenic bacteria (<1 mm). Results of phytochemical testing confirmed the result of inhibitory testing where macerated extract contained alkaloids, saponins, steroids, terpenoids, and tannins, while the leaf infusion only contained alkaloids and terpenoids. It can be concluded that macerated extracts of P. foetida leaves were far more effective in inhibiting the growth of S. aureus and E. coli. The study implies that the leaf extract of P. foetida can be considered and developed into a strong antibacterial in the future through good and appropriate bioprospecting.
Co-Authors CHRISLAVENA SHINTA DIRKS HALIDAH RAHAWARIN MELDA YUNITA MELDA YUNITA MUHAMMAD ZEIN ELFITRASYA NOOR ANDRYAN ILSAN ONY WIBRIYONO ANGKEJAYA SUKMAWATI SUKMAWATI