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All Journal Biodiversitas Journal of Biological Diversity
BENZHONG FU
Hubei Province Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, College of Life Science and Technology, Hubei Engineering University. Xiaogan, Hubei 432000, China
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR CAO ZHENG; JIEQIAN ZHU; XUWANG YANG; BENZHONG FU
Biodiversitas Journal of Biological Diversity Vol. 23 No. 11 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231118

Abstract

Abstract. Zheng C, Zhu J, Yang X, Fu B. 2022. Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR. Biodiversitas 23: 5681-5686. Walnut blight is caused by the bacterium Xanthomonas arboricola pv. juglandis (Xaj), is one of the most serious diseases in walnut production around the world. Understanding the genetic diversity of walnut blight pathogen is of great significance to developing a scientific and reasonable prevention and control strategy. The rep-PCR (Repetitive Sequence Polymerase Chain Reaction) technique is a powerful tool for analyzing plant pathogens. In this paper, using three pairs of rep-PCR universal primers (ERIC, BOX, and REP), a genetic diversity analysis was carried out on walnut blight-associated bacteria mainly isolated from different counties of Hubei Province, China. A study of 60 walnut blight-associated bacteria isolated in 2016 found that they had a high genetic similarity among them. There is no clear correlation between the genetic diversity of these isolates and their geographical or tissue origins. Interestingly, these clusters can also distinguish copper-resistant isolates from sensitive ones. Therefore, rep-PCR is a powerful tool to study the population of walnut pathogenic bacteria.
Co-Authors CAO ZHENG JIEQIAN ZHU XUWANG YANG