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FARAH AISYAH NAFIDIASTRI
Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions SALAMUN SALAMUN; RIZKY DANANG SUSETYO; FARAH AISYAH NAFIDIASTRI; RIZKI AMALIAH ZAIN; ROSSY PERMATA SARI; ALMANDO GERALDI; FATIMAH FATIMAH; NI’MATUZAHROH NI’MATUZAHROH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231054

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Abstract. Salamun, Susetyo RD, Nafidiastri FA, Zain RA, Sari RP, Geraldi A, Fatimah, Ni’matuzahroh. 2022. Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions Biodiversitas 23: 5431-5438. This research was carried out for molecular identification, as well as the determination and mechanism of action of larvicidal toxicity of Bacillus sp. EG6.4 isolated from breeding sites of Aedes aegypti from Gresik, East Java, Indonesia. Bacillus sp. EG6.4 was a Gram-positive endospore-forming bacteria. Molecular species identification using 16S rRNA gene sequencing showed that isolate had 97.89% similarity with Bacillus mojavensis. The isolate showed larvicidal toxicity against A. aegypti larvae. The lethal concentration 50% (LC50) values ??at 24 and 48hours exposure were 8.99±1.01 ×107 cells/mL and 8.43±1.01 ×107 cells/mL, respectively, while lethal time 50% (LT50) value was 11.9±1.1 hours. Production of chitinolytic enzymes or biosurfactants, chitinolytic and hemolytic assays were conducted to determine the larvicidal mechanism. As a result, Bacillus sp. EG6.4 showed hemolytic, but not chitinolytic activity, indicating its potency to produce biosurfactants. Transmission Electron Microscopy (TEM) result showed that isolate had oval-shaped endospores located subterminal with massive-shape parasporal inclusions. The detection of srfA-D gene showed that isolate produced surfactin biosynthesis thioesterase. Thus, Bacillus sp. EG6.4 produced biosurfactant that potentially to be developed as a biocontrol agent for disease vectors and plant pathogens.
The potential of indigenous Bacillus sp. BT3.1 as an antifungal biopesticide against the plant pathogen Fusarium oxysporum FARAH AISYAH NAFIDIASTRI; NATASYA THESSA LOLITA; HANA SHAFIYAH RIQINA; BELLA ZEZARI AURORA; ALMANDO GERALDI; TRI NURHARIYATI; AGUS SUPRIYANTO; NI’MATUZAHROH NI’MATUZAHROH; FATIMAH FATIMAH; SALAMUN SALAMUN
Biodiversitas Journal of Biological Diversity Vol. 25 No. 6 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250638

Abstract

Abstract. Nafidiastri FA, Lolita NT, Riqina HS, Aurora BZ, Geraldi A, Nurhariyati T, Supriyanto A, Ni’matuzahroh, Fatimah, Salamun. 2024. The potential of indigenous Bacillus sp. BT3.1 as an antifungal biopesticide against the plant pathogen Fusarium oxysporum. Biodiversitas 25: 2679-2686. Biosurfactants are green surfactants that bacteria can produce, one of which is Bacillus. This genus can produce lipopeptide-type biosurfactants, namely surfactin, that can inhibit the growth of fungal pathogens. This research aims to determine the biosurfactant activity on molasses substrates and the antifungal activity of isolate BT3.1 against Fusarium oxysporum. The biosurfactant activity test method tested hemolytic activity using the spot method, surface tension analysis, and emulsification activity. In addition, an antifungal activity test was also carried out using the swab method. Characterization of isolate BT3.1 was carried out macroscopically and microscopically. The macroscopic characteristics of isolate BT3.1 show that bacteria from the genus Bacillus are moderate in size, white, irregular with serrate colony edges, shiny raised elevations, and a shiny surface texture. Microscopic characteristics of the isolate showed Gram-positive bacteria with oval-shaped endospores in the center. Based on the results of the biosurfactant activity test, isolate BT3.1 showed beta-type hemolysis activity, reduced surface tension by 23.3 mN/m on distilled water and 12 mN/m on NB media, and produced an emulsion activity value of around 52%. The results of the production of crude extract of BT3.1 isolate on 2, 4, and 6% molasses substrates showed optimal biosurfactant activity at a concentration of 4% with an incubation time of 48 hours. The antifungal activity of isolate BT3.1 showed that 80% inhibited the growth of Fusarium oxysporum, so the indigenous bacteria Bacillus sp. BT-3.1 has the potential to be developed as a raw material candidate for antifungal biopesticides against the plant pathogen Fusarium oxysporum.
Co-Authors AGUS SUPRIYANTO ALMANDO GERALDI ALMANDO GERALDI BELLA ZEZARI AURORA FATIMAH FATIMAH FATIMAH FATIMAH HANA SHAFIYAH RIQINA NATASYA THESSA LOLITA NI’MATUZAHROH NI’MATUZAHROH NI’MATUZAHROH NI’MATUZAHROH RIZKI AMALIAH ZAIN RIZKY DANANG SUSETYO ROSSY PERMATA SARI SALAMUN SALAMUN SALAMUN SALAMUN TRI NURHARIYATI