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All Journal Biodiversitas Journal of Biological Diversity
WAHYU WIDORETNO
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Macroepiphyte biodiversity on Kappaphycus alvarezii surface and its interaction with environment in cultivation centers on Lombok Island, Indonesia MURSAL GHAZALI; WAHYU WIDORETNO; ESTRI LARAS ARUMINGTYAS; CATUR RETNANINGDYAH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 12 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231224

Abstract

Abstract. Ghazali M, Widoretno W, Arumingtyas EL, Retnaningdyah C. 2022. Macroepiphyte biodiversity on Kappaphycus alvarezii surface and its interaction with environment in cultivation centers on Lombok Island, Indonesia. Biodiversitas 23: 6284-6292. The aims of this paper are to investigate the influence of environmental factors on the diversity of macroepiphytes in Kappaphycus alvarezii. Macroepiphyte sampling was carried out at four cultivation centers: Seriwe Bay, Ekas Bay, Gerupuk Bay, and Siwak of Lombok Island, West Nusa Tenggara, Indonesia. Several stations were placed at each location, and at each station were used one to three cultivation units with five replications. Environmental data measurements were carried out at the time of sampling. Some parameters were measured: pH, temperature, salinity, current velocity, brightness, DO, BOD, nitrate, phosphate, and TSS. Data analysis was performed using several diversity indices. In addition, a biplot analysis was performed using PCA to analyze the correlation between environmental factors and environmental factors to macroepiphytes. The results showed that the most dominant species was Polysiphonia spaerocharpa, and the second was Ceramium sp1. The highest richness and abundance were found in Ekas Bay. The highest species dominance was in Siwak. The highest diversity index was in Ekas Bay. The correlation between environmental factors showed that hemeroby harmed depth, phosphate, and DO and positively affected BOD and the thallus surface structure. The principal correlation analysis showed that the abundance of macroepiphyte was influenced by BOD, the roughness of host thallus, and the hemeroby index.
In vitro mutagenesis on patchouli (Pogostemon cablin Benth.) with gamma-ray irradiation on leaf explants RUT NORMASARI; ESTRI LARAS ARUMINGTYAS; RURINI RETNOWATI; WAHYU WIDORETNO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 12 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241201

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Abstract. Normasari R, Arumingtyas EL, Retnowati R, Widoretno W. 2023. In vitro mutagenesis on patchouli (Pogostemon cablin Benth.) with gamma-ray irradiation on leaf explants. Biodiversitas 24: 6407-6414. Patchouli oil production in Indonesia has yet to meet international market demand, one of which is the quality of the patchouli superior seeds. Patchouli is propagated vegetatively through cuttings, causing limited variability and decreasing plant quality. In vitro mutagenesis with gamma-ray irradiation can increase plant genetic variability and produce superior patchouli plants. This study aimed to produce patchouli variants through in vitro mutagenesis by gamma-ray irradiation on patchouli leaf explants. To accomplish the research objectives mentioned above, the research stages included in vitro mutagenesis through gamma-ray irradiation, shoot regeneration, plantlet growth, and molecular analysis using SSR (Simple Sequence Repeat). Gamma-ray irradiation inhibited the growth of explants and shoot formation but could increase plantlet growth ability at low doses of 15-30 Gy. The dose of gamma-ray irradiation that caused the death of explants in fifty percent of the tested population (LD50) was 69 Gy. Molecular analysis of SSR on 50 plants regenerated from gamma-irradiated explants revealed monomorphic and polymorphic fragments compared to non-irradiated and donor plants. Primers Pca1 and Pca2 showed the highest percentage of polymorphic, with 77.3 and 50%, respectively. Gamma irradiation during in vitro culture is an alternate method for increasing genetic variety in patchouli breeding.
Phytochemicals’ compound and antioxidant activities in the food of Pongo tapanuliensis from Batang Toru Forest, North Sumatra, Indonesia HERNA FEBRIANTY SIANIPAR; WAHYU WIDORETNO; LUCHMAN HAKIM; REZI RAHMI AMOLIA; FATCHIYAH FATCHIYAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 10 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251007

Abstract

Abstract. Sianipar HF, Widoretno W, Hakim L, Amolia RR, Fatchiyah F. 2024. Phytochemicals’ compound and antioxidant activities in the food of Pongo tapanuliensis from Batang Toru Forest, North Sumatra, Indonesia. Biodiversitas 25: 3454-3463. Campnosperma auriculatum (Blume) Hook.fil. and Agathis borneensis Warb. are food for Pongo tapanuliensis Nurcahyo, Meijaard, Nowak, Fredriksson & Groves, 2017 found in the Batang Toru Forest, Indonesia. Studying the phytochemical properties of both plant species is important part of conservation framework for Tapanuli orangutans because information regarding their nutrient contents and benefits still needs to be investigated. This research examined the closest components, amino acids, and phytochemicals compounds that have antioxidant activity. The C. auriculatum and A. borneensis fruit were tested for proximate, amino acid, phytochemical, total flavonoid content, LC-MS/MS, and FRAP to determine antioxidant activity. The results of proximate and amino acid tests showed that C. auriculatum has higher contents of carbohydrates (74.49%), protein (7.54%), lipids (3.73%), energy from fat (33.57Kcal/100 g), total energy (361.71Kcal /100g), L-Histidine (1704.975ppm), L-Leucine (3759.28ppm), and L-Lysine (2099.038ppm) compared to A. borneensis. The phytochemical tests of both samples were positive for containing alkaloids, flavonoids, saponins, steroids, tannins, phenolics, and quinolines. The higher flavonoid content is found in C. auriculatum (94.44±2.77mg QE/g). LC-MS/MS results for both samples, C. auriculatum that is (3alpha,20R,24Z)-3-Hydroxy-21-oxoeupha-8,24-dien-26-oic acid, terpenoid group, and A. borneensis, that is Procyanidin B2, the flavonoid group. The higher antioxidant capacity was found in C. auriculatum (4.63±0.111µg/mL). The C. auriculatum has excellent potential as a natural source of nutrition and antioxidants for the Tapanuli orangutan; therefore, it is necessary to develop a strategy by cultivating these two plants and using them in pharmacognosy for P. tapanuliensis.
Co-Authors CATUR RETNANINGDYAH ESTRI LARAS ARUMINGTYAS FATCHIYAH FATCHIYAH HERNA FEBRIANTY SIANIPAR LUCHMAN HAKIM MURSAL GHAZALI REZI RAHMI AMOLIA RURINI RETNOWATI RUT NORMASARI