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All Journal Biodiversitas Journal of Biological Diversity
DWI PRIYOWIDODO
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Ancylostomiasis in cats in Yogyakarta, Indonesia, and its causative genetic relations JUNI CLAUDIA DAMI; LUH PUTU EKA DAMAYANTI; SOEDARMANTO INDARJULIANTO; DWI PRIYOWIDODO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240512

Abstract

Abstract. Dami JC, Damayanti LPE, Indarjulianto S, Priyowidodo D. 2023. Ancylostomiasis in cats in Yogyakarta, Indonesia, and its causative genetic relations. Biodiversitas 24: 2605-2611. Ancylostomiasis is a zoonotic cat disease caused by one or more species of Ancylostoma spp. This study aims to determine ancylostomiasis in cats in Yogyakarta and its causative genetic relations. This research used ten cats with ancylostomiasis, based on the findings of Ancylostoma spp. eggs in their feces. Cats were examined clinically and laboratory, including blood profile and identification of Ancylostoma spp. eggs. The ITS1-5.8S-ITS2 region of Ancylostoma spp. was amplified by polymerase chain reaction (PCR) and sequenced; the results were compared with the Basic Local Alignment Search Tool (BLAST). As a result, physical examination showed that cats with ancylostomiasis suffered from diarrhea (100%), decreased appetite (70%), weakness (60%), anemia (60%), emaciation, and dull hair (10%). Blood examination showed that 5 (50%) cats had normochromic normocytic anemia, and 1 (10%) cat had hypochromic microcytic anemia. PCR tests, sequencing, and phylogenetic tests found that Ancylostoma spp. isolates had been successfully identified as Ancylostoma braziliense De Faria 1910, identical to A. braziliense from Australian isolate (DQ359149.1). Therefore, it was concluded that ancylostomiasis in cats in Yogyakarta was caused by A. braziliense.
Detection of Plasmodium in small ruminants in Yogyakarta, Indonesia, using a nested PCR assay DWI PRIYOWIDODO; APRIL HARI WARDHANA; DYAH HARYUNINGTYAS SAWITRI; JOKO PRASTOWO; WISNU NURCAHYO; AAN AWALUDIN; YUDHI RATNA NUGRAHENI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 12 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241234

Abstract

Abstract. Priyowidodo D, Wardhana AH, Sawitri DH, Prastowo J, Nurcahyo W, Awaludin A, Nugraheni YR. 2023. Detection of Plasmodium in small ruminants in Yogyakarta, Indonesia, using a nested PCR assay. Biodiversitas 24: 6722-6726. Limited investigation of natural malaria infection in small ruminants in Yogyakarta needs to be explored. Plasmodium sp., as the causative agent of malaria, has been proven to infect ungulates, including small ruminants. This study aimed to investigate the presence of natural malaria infection in goats and sheep in Yogyakarta. A total of 303 blood samples were collected from goats and sheep in this study from four selected areas by a cross-sectional study. A microscopic examination detected the Plasmodium stage in a thin blood smear. Nested Polymerase Chain Reaction (PCR) performed molecular detection, targeting cytochrome b (cytb) as a gene target. It was found that no Plasmodium stage was detected in thin blood smears. However, three out of 70 blood samples from Girimulyo were Plasmodium-positive based on nested PCR assay. The results highlight that molecular assay by nested PCR is more sensitive than microscopic examination. Molecular analysis revealed natural Plasmodium infections in Ettawa cross-bread goats, with a proportion of 0.0429 (95% CI: 0.0089-0.1202). There was no statistical difference between sex and natural malaria infection in goats. This finding suggests that molecular analysis is necessary to detect natural malaria infection with extremely low parasitemia levels. Therefore, further study with larger sample sizes and broader geographical representation is needed to fully understand these malaria infections in goats and sheep.
Molecular detection and hematological profile of Trypanosoma evansi in livestock NILA QUDSIYATI; RADEN WISNU NURCAHYO; DWI PRIYOWIDODO; SOEDARMANTO INDARJULIANTO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 9 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250937

Abstract

Abstract. Qudsiyati N, Nurcahyo RW, Priyowidodo D, Indarjulianto S. 2024. Molecular detection and hematological profile of Trypanosoma evansi in livestock. Biodiversitas 25: 3154-3159. Trypanosoma evansi, which assaults a variety of hosts and is found around the world, and is the causative agent of Surra sickness. The greatest Surra outbreak occurred in Indonesia in 2010-2012, primarily in Sumba, killing about 2,000 animals. The research purpose is to confirm T. evansi infection in livestock and determine hematological parameters in naturally infected livestock compared to non-infected (control). This study used molecular and parasitological testing techniques to identify Surra illness in the field. The buffy coat technique, Polymerase Chain Reaction (PCR), and thin blood smear test are used in the research methodology. Hematological parameters (red blood cell count, hemoglobin concentration, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, red cell distribution width, and white blood cell count) were analyzed. According to the PCR results, out of 222 blood samples, only 1 horse blood sample from Sumba shows a positive result for T. evansi. PCR is the most effective diagnostic tool for trypanosomiasis. This was the first time T. evansi DNA was discovered in Konga Loko Hamlet, Karuni Village, Sumba, Indonesia.
Co-Authors AAN AWALUDIN APRIL HARI WARDHANA DYAH HARYUNINGTYAS SAWITRI JOKO PRASTOWO JUNI CLAUDIA DAMI LUH PUTU EKA DAMAYANTI NILA QUDSIYATI RADEN WISNU NURCAHYO SOEDARMANTO INDARJULIANTO WISNU NURCAHYO YUDHI RATNA NUGRAHENI