HANI PLUMERIASTUTI
Division of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia

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First report of pentastomiasis and the lung histopathological changes in Oriental Rat Snake (Ptyas mucosa) in Sidoarjo, East Java, Indonesia HANI PLUMERIASTUTI; GARINDRA TIARA PRANASHINTA; LUCIA TRI SUWANTI; ANNISE PROBONINGRAT; KUSNOTO KUSNOTO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240414

Abstract

Abstract. Plumeriastuti H, Pranashinta GT, Suwanti LT, Proboningrat A, Kusnoto. 2023. First report of pentastomiasis and the lung histopathological changes in Oriental Rat Snake (Ptyas mucosa) in Sidoarjo, East Java, Indonesia. Biodiversitas 24: 2045-2051. Currently, reptiles, especially snakes, are increasingly popular as pets worldwide and are often consumed by several communities in Indonesia. This trend needs attention from a public health perspective because it can potentially cause zoonotic diseases, including parasitic disease such as pentastomiasis. This study aimed to determine: the prevalence of pentastomiasis, identify the parasite and observe the pulmonary histopathology in Oriental Rat Snakes (Ptyas mucosa Linnaeus 1758) collected in Tulangan, Sidoarjo, Indonesia. The methods used were calculating the prevalence of pentastomiasis, semichen-acetic carmine staining, and identifying parasites and eggs based on references as a comparison. Furthermore, histopathological preparation and hematoxylin-eosin staining were performed on the lungs infected with the parasite to observe the microscopic lesions. The results showed that 65% of the snakes had pentastomiasis, and the parasites were called Kiricephalus pattoni Stephens 1908. Furthermore, a microscopical examination revealed mild fibrosis in the pulmonary area due to pentastomid infestation. This study is the first reported pentastomida in P. mucosain Sidoarjo, Indonesia, showing that snakes caught and commonly consumed were infected with zoonotic pentastomic.
Phytochemical analysis and antioxidant activities ethanol extract of propolis Trigona spp. from different vegetation in Lombok, West Nusa Tenggara, Indonesia KUNTI TIRTASARI; LUCIA TRI SUWANTI; MUFASIRIN MUFASIRIN; POEDJI HASTUTIEK; ELLY NUR INDASARI; ROCHMAH KURNIJASANTI; HANI PLUMERIASTUTI; ERMA SAFITRI; EKA PRAMYRTHA HESTIANAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250147

Abstract

Abstract. Tirtasari K, Suwanti LT, Mufasirin, Hastutiek P, Indasari EN, Kurnijasanti R, Plumeriastuti H, Safitri E, Hestianah EP. 2024. Phytochemical analysis and antioxidant activities ethanol extract of propolis Trigona spp. from different vegetation in Lombok, West Nusa Tenggara, Indonesia. Biodiversitas 25: 404-411. Propolis is a natural substance containing a resin that is collected by honey bees from various plants and has a significant effect on human health such as antibacterial, antifungal, anti-inflammatory, antiviral, anesthetic, antioxidant, antitumoural, antiprotozoal, anticancer, antihypertensive, anticarcinogenic, antihepatotoxic and has cytotoxic activity. Cultivation of Trigona spp. in Lombok is growing rapidly. Trigona spp. produces more propolis than honey bees of Apis spp., but bee products that are maximally utilized are only honey, and propolis has not been utilized optimally. Currently, there is no scientific validation of the phytochemical content, bioactive compounds, and biological activities of ethanol extract propolis Trigona spp. from Lombok, West Nusa Tenggara. So, this study aimed to determine the phytochemical compounds, bioactive compounds, and antioxidant activity of propolis. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to determine the bioactive compounds of ethanol extract propolis. DPPH (2,2-diphenyl-1-picrylhydrazyl) was used to evaluate antioxidant activity. The composition of propolis compounds is different for each propolis depending on the bee species and the vegetation source from which the resin was collected. In this research, propolis was collected from different vegetation sources (cashews, moringa, coffee, and lote trees). This study used qualitative and quantitative phytochemical analysis and the antioxidant DPPH test. Different components and percentages cause significant variations in the results obtained from phytochemical tests. The content is different in various propolis, there are saponins, flavonoids, and tannins. The highest antioxidant activity was found in propolis with moringa resin 38.4150 (very strong), followed by lote tree resin -66.0420 (strong), cashew resin 81.8140 (strong), and coffee resin 100.7465 (moderate).