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All Journal Biodiversitas Journal of Biological Diversity
WARAS NURCHOLIS
Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Agatis, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: Differences in phytochemical compounds and antioxidant activity of Portulaca oleracea and Portulaca grandiflora SYARIFAH IIS AISYAH; ALDILA WIDYA PUTRI OKTAVIA; AULIA ARDHIAN AYUNINGTYAS; RAIHAN PERMANA PUTRA; SELNIS PRASSISKA; SITI JAMILAH; WARAS NURCHOLIS
Biodiversitas Journal of Biological Diversity Vol. 24 No. 3 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240307

Abstract

Abstract. Aisyah SI, Oktavia AWP, Ayuningtyas AA, Putra RP, Prassiska S, Jamilah S, Nurcholis W. 2023. Short Communication: Differences in phytochemical compounds and antioxidant activity of Portulaca oleracea and Portulaca grandiflora. Biodiversitas 24: 1385-1390. Portulaca oleracea and Portulaca grandiflora are medicinal ornamental plants used in traditional and modern medicine. Therefore, this study aimed to analyze the phytochemical compounds and antioxidant activity in two purslane plants, P. oleracea,and P. grandiflora. The purslane ethanol extract was obtained by sonication and maceration methods. The phytochemical screening included the examination of secondary plant metabolites, while antioxidant activity was analyzed using the 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and cupric ion reducing antioxidant capacity (CUPRAC) methods. The results showed that both extract samples contain alkaloids, phenol hydroquinones, flavonoids, saponins, tannins, triterpenoids, and steroids. The highest ABTS antioxidant activity was found in P. grandiflora extract at 26.93 µmol TE/g FW compared to P. oleracea extract at 15.98 µmol TE/g FW. The highest CUPRAC antioxidant activity was found in the P. oleracea extract at 33.22 µmol TE/g FW, while in the P. grandiflora extract, it was 18.55 µmol TE/g FW. Based on the results, it was concluded that the chemical complexity of various purslane plants causes differences in the antioxidant capacity.
Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes RAISADIBA PUTRI BOVANI; NOVIAN LIWANDA; IRMANIDA BATUBARA; LAKSMI AMBARSARI; WARAS NURCHOLIS
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250236

Abstract

Abstract. Bovani RP, Liwanda N, Batubara I, Ambarsari L, Nurcholis W. 2024. Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes. Biodiversitas 25: 763-769. Orthosiphon aristatus (Blume) Miq., also known as kumis kucing in Indonesia, is a medicinal plant belonging to the Lamiaceae family that contains various phytochemical compounds with antioxidant properties. This study investigated the phenolic content, flavonoid, rosmarinic acid,  sinensetin,  and antioxidant capacity of 15 genotypes of O. aristatus ethyl acetate extracts. Therefore, 15 genotypes were analyzed using quercetin as the standard for Total Phenolic Content (TPC) using a colorimetric method with gallic acid and Total Flavonoid Content (TFC). Sinensentin and rosmarinic acid were measured by High-Performance Liquid Chromatography (HPLC). Antioxidant capacity was determined using Trolox as a standard antioxidant by 2-Diphenyl Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays. The results revealed a diverse range of phytochemical compounds, with total phenolic content ranging from 3.39 to 13.80 mg GAE/g DW, total flavonoid content from 0.20 to 1.43 mg QE/g DW, rosmarinic acid from 0.02 to 0.19 mg/g DW, and sinensetin from 1.61 to 21.67 mg/g DW. Furthermore, the antioxidant assays demonstrated varying degrees of efficacy, with DPPH ranging from 2.09 to 13.98 ?mol TE/g DW and FRAP from 0.07 to 2.96 ?mol TE/g DW. Genotypes A10, A11, and A12 exhibited superior phytochemical contents and antioxidant capacities, highlighting their potential as valuable sources of medicinal compounds.
Co-Authors ALDILA WIDYA PUTRI OKTAVIA AULIA ARDHIAN AYUNINGTYAS IRMANIDA BATUBARA LAKSMI AMBARSARI NOVIAN LIWANDA RAIHAN PERMANA PUTRA RAISADIBA PUTRI BOVANI SELNIS PRASSISKA SITI JAMILAH SYARIFAH IIS AISYAH