ABIL DERMAIL
Department of Agronomy, Faculty of Agriculture, Khon Kaen University. Khon Kaen 40002, Thailand

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Stratified haploid identification system through the R1-nj kernel and reduced seedling vigor in tropical maize germplasm ARNAT THAWARORIT; ABIL DERMAIL; KAMOL LERTRAT; SOMPONG CHANKAEW; KHUNDEJ SURIHARN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 8 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240807

Abstract

Abstract. Thawarorit A, Dermail A, Lertrat K, Chankaew S, Suriharn K. 2023. Stratified haploid identification system through the R1-nj kernel and reduced seedling vigor in tropical maize germplasm. Biodiversitas 24: 4262-4268. Haploid identification through the R1-nj marker is commonly applied in in-vivo doubled haploid technology in maize, although misclassification issues often occur. We explored the innate properties of maternal haploids at the seedling stage to verify true haploids and to reduce false positives. There are many questions about whether reduced seedling vigor is effective for haploid verification in tropical maize backgrounds. This study aimed to evaluate the haploid induction rate of Stock6-derived F3 population inducers and to investigate the effectiveness of reduced seedling vigor at the V2/V3stage as an alternative marker to verify putative haploids. Field trials and haploid induction were conducted in the rainy season of 2021 and the dry season of 2021/22 in Khon Kaen, Thailand. Two tropical source germplasm: P789 and S7328, were included as donor females. Haploids were identified through the R1-nj marker and reduced seedling vigor. Significant reduction of haploid frequency up to 2.5% on average between the two identification methods, indicating that a considerable number of false positives could be found using reduced seedling vigor. Inducer population K11 was promising since it performed a stable ability to produce haploids over donors and seasons and had a low misclassification rate. Further breeding strategies and haploid selection schemes are discussed.
Reciprocal cross effect over seasons on agronomic and yield traits in waxy corn under tropical savanna MATTANA SAWANGHA; ABIL DERMAIL; SOMPONG CHANKAEW; SAKUNKAN SIMLA; KAMOL LERTRAT; KHUNDEJ SURIHARN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 7 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240750

Abstract

Abstract. Sawangha M, Dermail A, Chankaew S, Simla S, Lertrat K, Suriharn K. 2023. Reciprocal cross effect over seasons on agronomic and yield traits in waxy corn under tropical savanna. Biodiversitas 24: 4120-4125. The exponentially increasing number of hybrids per additional inbred lines results in a tremendous workload in crop hybrid breeding. The question arises if reciprocal crosses could be excluded in waxy corn hybrids to reduce the workload by up to 50%. In this study, we aimed to assess the importance of reciprocal cross effects for yields, yield components, and agronomic traits of sweet-waxy corn F1hybrids and to validate the stability of those effects over seasons. Five parental lines, comprised of one sweet corn inbred line and four waxy corn inbred lines, were mated through a top cross scheme to generate eight F1progenies, including the reciprocals. All genotypes, including checks, were arranged in a Randomized Complete Block Design (RCBD) with three replications in the dry season of 2021/2022 and the rainy season of 2022 in Khon Kaen, Thailand. Genotype was significant for all observed traits, and the interaction between genotype and season was significant for all traits. The reciprocal cross-effect was non-significant for most observed traits. The non-significance of the interaction between N vs. R and season confirmed the absence of reciprocal cross effects in different growing seasons. Thus, dissecting one of two possibilities in each cross combination is advisable for affordable hybrid formations. Considering the absence of reciprocal cross effects but significant G × E effect for given traits, allocating more efforts and resources for conducting multi-environment trials is advisable to reduce the gap between actual and potential yields of testing hybrids.