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All Journal Biodiversitas Journal of Biological Diversity
JUANDY JO
Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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A comparative assessment of Lactiplantibacillus plantarum isolated from chicken and humans as candidates for probiotics JESSICA SUNARDI; EMILY TANIA PURNAMA; MARCELIA SUGATA; HANS VICTOR; TAN TJIE JAN; JUANDY JO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240964

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Abstract. Sunardi J, Purnama ET, Sugata M, Victor H, Jan TT, Jo J. 2023. A comparative assessment of Lactiplantibacillus plantarum isolated from chicken and humans as candidates for probiotics. Biodiversitas 24: 5198-5206. Lactiplantibacillus plantarum is commonly analyzed as a potential probiotic. We hereby investigated two strains isolated from chicken crop (Lpb. plantarum F75) and human breast milk (Lpb. plantarum SU-KC1a). Ability to withstand osmotic stress (1.5%, 2.5% or 3.5% of NaCl) and phenol compounds (0.2% or 0.5%), ability to survive gastric juices for a maximum of 120 minutes and bile salt for a maximum 3 hours, as well as susceptibility to 25 antibiotic discs, were compared between both strains. Whole genomes of both strains were sequenced and analyzed in silico to determine the availability of antibiotic-resistance genes as well as the presence of mobile genetic elements and plasmid. Both strains were sensitive to increased concentrations of NaCl and phenol as well as to prolonged exposure to gastric juices. In contrast, both strains could withstand a prolonged exposure of 0.3% of bile salt. Both isolates had similar genome sizes and were susceptible to many tested antibiotics. The detected resistance genes were observed within the chromosomal genomes but no mobile genetic element nor plasmid was found. In conclusion, both strains of Lpb. plantarum displayed several characteristics of beneficial bacteria and could be used as probiotic candidates for poultry and human beings, respectively.
Physiological and molecular characterization of copper and antibiotic resistance mechanisms in Pseudomonas aeruginosa strain PaD2 JUANDY JO; DIAN ANGELICA TRYOANITA UMBU DATTA; WAHYU IRAWATI; JONATHAN SUCIONO PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260404

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Abstract. Jo J, Datta DATU, Irawati W, Purnomo JS. 2025. Physiological and molecular characterization of copper and antibiotic resistance mechanisms in Pseudomonas aeruginosa strain PaD2. Biodiversitas 26: 1526-1536. Environmental pollution by heavy metals and antibiotics promotes the growth of co-resistant bacteria that can spread multiple resistance genes. Bioremediation using indigenous bacteria from such polluted sites is a potential solution to this problem. We recently isolated Pseudomonas aeruginosa strain PaD2 from the polluted Sukolilo River in Surabaya, Indonesia, and found it to be resistant to high copper concentrations. Therefore, we investigated the resistance profiles of P. aeruginosa PaD2 to Cu and various antibiotics using physiological and molecular approaches. Antibiotic resistance was evaluated in the presence of 4 mM CuSO?, as compared to that without Cu addition, using disk diffusion and broth macrodilution assays. The whole genome of P. aeruginosa PaD2 was sequenced to identify relevant resistance genes. Pseudomonas aeruginosa PaD2 exhibits resistance to both copper and various antibiotics (e.g., cefoxitin, erythromycin or tetracycline). Interestingly, Cu exposure did not significantly alter the antibiotic resistance profiles, indicating that Cu and antibiotic resistance mechanisms were independent. Genome analysis identified genes related to efflux pumps, antibiotic inactivation, and target modification, which may explain the multidrug resistance phenotype. In conclusion, P. aeruginosa PaD2 should be further tested as a bioremediation agent for copper and antibiotic pollution.
Co-Authors DIAN ANGELICA TRYOANITA UMBU DATTA EMILY TANIA PURNAMA HANS VICTOR JESSICA SUNARDI JONATHAN SUCIONO PURNOMO MARCELIA SUGATA TAN TJIE JAN WAHYU IRAWATI