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All Journal Biodiversitas Journal of Biological Diversity
VU THI THUY HANG
Faculty of Agronomy, Vietnam National University of Agriculture. Trau Quy, Gia Lam, Hanoi 100000, Vietnam
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Genetic diversity of rose apple (Syzygium samarangense) varieties based on ISSR molecular markers PHAN THI THU HIEN; VU THI THUY HANG; LE THI TUYET CHAM
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250517

Abstract

Abstract. Hien PTT, Hang VTT, Cham LTT. 2024. Genetic diversity of Rose apple (Syzygium samarangense) varieties based on ISSR molecular markers. Biodiversitas 25: xxxx. Rose apple (Syzygium samarangense) exhibits a rich diversity in its varieties, metabolic compounds, and biological activities. Genetic diversity studies are crucial in understanding plant species taxonomy and evolutionary history. They provide a roadmap for researchers to trace the relationships between different varieties and offer insights into the evolutionary processes that have shaped the diversity within the species. Many traditional or indigenous varieties of plants may harbor unique traits adapted to specific local conditions. This study, therefore, holds immense value as it aims to evaluate the genetic relationship among rose apple varieties in Vietnam using morphological characteristics and ISSR markers. Sixteen local varieties of rose apple were collected from provinces in Vietnam for this purpose. The morphological characteristics of leaves and fruits were compared among samples, and the genetic diversity was evaluated using ISSR markers. The results revealed significant morphological diversity in the leaf base, blade shape, leaf color, and fruit shape and size. Based on the DNA profiles of 15 ISSR markers, 102 amplicons were produced, and the percentage of polymorphism ranged from 57 to 100%. The dendrogram constructed using the UPGMA method classified these varieties into two main clusters with a genetic diversity of 64%. Such comprehensive information underscores the genetic diversity among S. samarangense, thereby contributing to the germplasm resources for future plant breeding programs.
Genetic diversity of Guava varieties (Psidium guajava L.) based on morphology and ISSR molecular markers PHAN THI THU HIEN; LE THI TUYET CHAM; VU THI THUY HANG
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250317

Abstract

Abstract. Hien PTT, Cham LTT, Hang VTT. 2024. Genetic diversity of Guava varieties (Psidium guajava L.) based on morphology and ISSR molecular markers. Biodiversitas 25: 1037-1045. Guava (Psidium guajava L.) is a fleshy-fruited representative belonging to the Myrtaceae family that has attracted attention due to its economic benefits. In this study, seventeen guava varieties in the Mekong Delta and Hanoi were collected and genetically validated by both morphology and DNA markers. The results revealed that fruit shape is a powerful indicator of guava discrimination. There are 12 out of 15 Inter Simple Sequence Repeats (ISSR) markers that show informative band patterns and 93 amplicons are generated with the size in length 200-2000 bp. The average polymorphic band and PIC value are 97.85% and 36%, respectively. Based on the dendrogram constructed by the Unweighted Pair Group Method using arithmetic Averages (UPGMA) method, samples were grouped into two main groups and the similarity level ranged from 55-98%. Group 2 contains Oi Tim (Purple guava), a special variety that showed high differences in terms of morphology and DNA profiles. Such information reflects the diversity of guava germplasm in Viet Nam, which is useful for conservation, and breeding strategies. The result revealed that 11 varieties expressed genetic diversity through both phenotypic traits and DNA profiles. Such information illustrates the genetic diversity among Psidium guajava, contributing to germplasm resources and plant breeding programs.
Co-Authors LE THI TUYET CHAM PHAN THI THU HIEN PHAN THI THU HIEN