TUTY ARISURYANTI
Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia

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Taxonomic study on selected species of stingless bees (Hymenoptera: Apidae: Meliponini) in Sulawesi Island, Indonesia MANAP TRIANTO; TUTY ARISURYANTI; HARI PURWANTO; ROSICHON UBAIDILLAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250547

Abstract

Abstract. Trianto M, Arisuryanti T, Purwanto H, Ubaidillah R. 2024. Taxonomic study on selected species of stingless bees (Hymenoptera: Apidae: Meliponini) in Sulawesi Island, Indonesia. Biodiversitas 25: 2290-2306. Stingless bees are strong candidates as alternative crop pollinators. They are found throughout the tropics, with the exception of the Pacific islands. There are three tropical regions in the world, namely the tropical Americas (Neotropic), Sub-Saharan Africa (Afrotropic), and Indoaustralian (Austroasiatic) region. In this research, we explored the morphological and morphometric characteristics of stingless bees in Sulawesi Island, Indonesia. The genera and species identification of stingless bees were based on morphological characters and coloration, such as the structure and color of the antenna, head, thorax, wing, leg and abdomen. Morphological and morphometric identification of 10 species of stingless bees consisting of five genera, namely the genera Heterotrigona, Homotrigona, Lepidotrigona, Tetragonula, and Wallacetrigona. The genus Tetragonula consists of six small species (<4 mm), namely T. biroi, T. clypearis, T. fuscobalteata, T. laeviceps, T. pagdeni, and T. sapiens. The genera Heterotrigona, Homotrigona, Lepidotrigona, and Wallacetrigona are a group of large stingless bees (>4 mm), each consisting of one species, namely H. itama, H. canifrons, L. terminata, and W. incisa. H. itama and H. canifrons are new records on Sulawesi Island. These species are found in Lore Lindu National Park (TNLL). Until 2023, these species were only found in Java, Kalimantan and Sumatra. This research can be used as a guide in the identification and validation of stingless bee species on Sulawesi Island.
DNA barcoding of medaka fish Oryzias marmoratus in Lake Towuti, South Sulawesi, Indonesia REZKI ASYA NUR; ANDI PARENRENGI; TUTY ARISURYANTI; HELMY WIDYASTUTI; IRMA ANDRIANI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 10 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251026

Abstract

Abstract. Nur RA, Parenrengi A, Arisuryanti T, Widyastuti H, Andriani I. 2024. DNA barcoding of medaka fish Oryzias marmoratus in Lake Towuti, South Sulawesi, Indonesia. Biodiversitas 25: 3645-3652. Oryzias marmoratus (Aurich, 1935), an endemic species of Lake Towuti, the second-largest lake in Indonesia, is facing a critical situation. Its population in nature is near threatened, as per the IUCN, necessitating immediate molecular studies such as DNA barcoding techniques. These studies are crucial to prevent a decline in the population of O. marmoratus. DNA barcoding is not just a tool for identification, monitoring, and protection but a call for genetic conservation. Therefore, the research was intended to identify the genetic variation of medaka fish. O. marmoratus was collected in Lake Towuti, and for DNA barcoding using primers, the cytochrome oxidase subunit I (COI) gene. Medaka fish samples (n=8) were collected from fishermen. DNA was extracted, and nucleotides were sequenced using sequencing provider 1st Base. The sequences of DNA were analyzed using Bioedit, BLAST-N, Mega11, DnaSP, and Network programs to obtain a consensus sequence, haplotype diversity, nucleotide diversity, genetic distance, phylogenetic, and haplotype network. The result showed that in the BLAST-N analysis, eight samples were closely related to O. marmoratus (LC154797.1) (AP005981.1) (94,42-99,84% in similarity). Genetic variation indicated a high value with a detailed 0.983 haplotype diversity, 0.026 a low nucleotide diversity, and six haplotypes; genetic distance with an average of 0.025 and 0.079. Phylogenetic analysis established five clades. Information on intraspecies genetic variation of O. marmoratus in terms of genetics, especially in Lake Towuti, is needed for genetic conservation and as an animal model of biology and molecular studies.