SRI PUJI ASTUTI WAHYUNINGSIH
Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia

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Effect of Lactobacillus casei FNCC 0090 to improve gastrointestinal bacterial abundance, immune system and water quality in catfish farming NURUL ‘AINI; MUHAMMAD BACHRUDDIN; FATIMAH FATIMAH; SAPTO ANDRIYONO; SRI PUJI ASTUTI WAHYUNINGSIH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250531

Abstract

Abstract. ‘Aini N, Bachruddin M, Fatimah, Andriyono S, Wahyuningsih SPA. 2024. Effect of Lactobacillus casei FNCC 0090 to improve gastrointestinal bacterial abundance, immune system and water quality in catfish farming. Biodiversitas 25: 2130-2138. Catfish is the most widely cultivated type of fish. However, along with the increase in catfish production as an effort to fulfill the fishery stock, there is an obstacle to the emergence of disease infection caused by Aeromonas hydrophila. For this reason, good fisheries management is required. One of the efforts to deal with this is by giving probiotics Lactobacillus casei FNCC 00990. Lactobacillus casei FNCC 0090 is expected to improve the immune system of catfish through improving the blood profile of fish and increasing the number of lactic acid bacteria in the gastrointestinal tract of catfish. This research aims to determine the effect of administering the probiotic L. casei FNCC 0090 on the abundance of microbes in the fish digestive tract, immune system, and water quality in cultivating catfish infected with A. hydrophila. The research design was a completely randomized design with five treatments and five replications. The treatments were A (administering 5% probiotics and infected with 0.1 mL x 108 CFU/mL), B (10% probiotics and infected), C (15% probiotics and infected), KP (positive control with 0% probiotics and infected with pathogens), KN (negative control, without probiotics and pathogen infection). Fish are kept for 42 days; on the 35th day, the fish were infected with A. hydrophila by intramuscular injection. Probiotics were mixed with feed with a total of 108 CFU/mL. Data in the form of BAL counts and total bacterial counts of fish digestive tracts, blood profiles, and water quality were analyzed using Statistical Package for the Social Sciences (SPSS) software version 23.0 (IBM SPSS Statistics). The results showed
Analysis of two strain probiotics on digestive enzymes, liver function and antimicrobial activity of catfish (Clarias gariepinus) treated with Aeromonas hydrophila NURUL AINI; SRI PUJI ASTUTI WAHYUNINGSIH; FARAH NISA UZAKKY; CAROLINE BETZY; FATIMAH FATIMAH; SAPTO ANDRIYONO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 11 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251132

Abstract

Abstract. Aini N, Wahyuningsih SPA, Uzakky FN, Betzy C, Fatimah, Andriyono S. 2024. Analysis of two strain probiotics on digestive enzymes, liver function and antimicrobial activity of catfish (Clarias gariepinus) treated with Aeromonas hydrophila. Biodiversitas 25: 4333-4339. Catfish (Clarias gariepinus Burchell 1822) is one of the most consumed fish. Good fisheries management is needed to maintain fish stocks. One of the efforts to manage fisheries safely is by using probiotics in the form of Duo Strain Probiotics (DSP). DSP is a probiotic formula consisting of Lactobacillus casei and Bacillus subtilis. The purpose of this study was to determine the effect of DSP on digestive enzyme levels, liver health status, and the potential of catfish blood serum as an antimicrobial agent. The treatments included DSP to the feed with concentrations of 0%, 5%, 10% and 15% using Aeromonas hydrophila infection. The results showed no significant change in amylase enzyme level between DSP treatments of various concentrations and the control treatment. DSP treatment of 10% and 5% in the non-infected fish group was the best treatment for increasing the level of protease and lipase enzymes, respectively. For liver health status parameters, 15% DSP treatment was the best treatment that could suppress the production of AST and ALT enzymes in fish blood serum. Catfish blood serum infected with pathogens and treated with 15% DSP has the ability as an antibacterial agent against A. hydrophila.