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All Journal Biodiversitas Journal of Biological Diversity
PARLUHUTAN SIAHAAN
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi. Jl. Kampus Bahu, Manado 95115, North Sulawesi, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Characterization of Trichoderma sp. local isolate and antagonism assay against pathogen Helminthosporium sp. PARLUHUTAN SIAHAAN; AGUSTINA MONALISA TANGAPO; STELLA DEIBY UMBOH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 9 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250960

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Abstract. Siahaan P, Tangpao AM, Umboh SD. 2024. Characterization of Trichoderma sp. local isolate and antagonism assay against pathogen Helminthosporium sp. Biodiversitas 25: 3380-3389. Trichoderma, as a biological agent, plays an important role in controlling pathogens that cause plant diseases, so it needs to be utilized properly, especially for local isolates. This research aimed to analyze the morphological, molecular and secondary metabolic characteristics of local isolates of Trichoderma fungus and test their antagonism against the pathogenic fungus Helminthosporium sp. The results showed that Tomohon local isolate of Trichoderma was identified as T. asperellum. The results of GC-MS analysis show that T. asperellum produces secondary metabolites in the form of volatile compounds from the group of volatile aldehydes, esters and ketones, as well as four fatty acid compounds, namely n-hexadecanoic acid, hexadecanoic acid, methyl ester, octadecadienoic acid, methyl ester, 9,12-octadecadienoic acid (Z,Z), play an important role in the antagonistic ability of Trichoderma. T. asperellum was able to inhibit Helminthosporium sp. by 70% on the seventh day of observation, and had antagonistic mechanisms, namely antibiosis and competition. Based on these results, local isolates of T. asperellum have great potential for use as biological control agents.
Characterization, molecular and pathogenicity of Metarhizium huainamdangense isolated from Dumoga against the corn leafhopper Peregrinus maidis PARLUHUTAN SIAHAAN; MAGFIRA LANGANAWA; ROWLAND ERVAN RUSFANDI MANGAIS
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261213

Abstract

Abstract. Siahaan P, Langanawa M, Mangais RER. 2025. Characterization, molecular and pathogenicity of Metarhizium huainamdangense isolated from Dumoga against the corn leafhopper Peregrinus maidis. Biodiversitas 26: 6111-6119. The corn leafhopper (Peregrinus maidis) is a major pest of maize, causing significant yield losses and threatening sustainable production. Reliance on chemical pesticides has led to resistance and environmental risks, creating a demand for eco-friendly alternatives. Entomopathogenic fungi, particularly Metarhizium spp., have shown strong potential as biological control agents. This study characterized and evaluated the pathogenicity of a local Metarhizium isolate from West Dumoga, North Sulawesi, Indonesia. Morphological observations confirmed colony and conidial traits typical of Metarhizium. Molecular identification through ITS sequencing placed the isolate within the Metarhizium huainamdangense clade with >98% similarity. Conidial density reached 1.39 × 10? conidia/mL with 100% germination after 24 hours, indicating strong physiological fitness. Pathogenicity assays against P. maidis demonstrated concentration- and time-dependent mortality, with up to 100% mortality at 10? conidia/mL by day 14. However, the high LC?? (1 × 10?. conidia/mL) and LT?? (>8.5 days) values indicate relatively low virulence compared with other Metarhizium species. This study confirms the Dumoga local isolate as Metarhizium huainamdangense and has potential as a promising biocontrol agent with formulation optimization to improve field effectiveness in integrated pest management.
Co-Authors AGUSTINA MONALISA TANGAPO MAGFIRA LANGANAWA ROWLAND ERVAN RUSFANDI MANGAIS STELLA DEIBY UMBOH