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All Journal Biodiversitas Journal of Biological Diversity
REGINAWANTI HINDERSAH
Department of Soil Science and Land Resources, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Enhanced mycorrhiza helper bacterial inoculant for improving the health of Arabica coffee seedlings grown in nematode-infected soil REGINAWANTI HINDERSAH; IIS NUR ASYIAH; WIDI AMARIA; BETTY NATALIE FITRIATIN; IMAM MUDAKIR; SAON BANERJEE
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260114

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Abstract. Hindersah R, Asyiah IN, Amaria W, Fitriatin BN, Mudakir I, Banerjee S. 2025. Enhanced mycorrhiza helper bacterial inoculant for improving the health of Arabica coffee seedlings grown in nematode-infected soil. Biodiversitas 26: 127-124. The Arbuscular Mycorrhizal Fungi (AMF) and Mycorrhiza Helper Bacteria (MHB) combine to combat the Pratylenchus coffeae nematode infection on coffee plantations sustainably and synergistically. Additionally, AMF facilitates the availability of phosphorus in plants. The objectives of present study are to formulate an enhanced MHB liquid inoculant containing Bacillus subtilis and Pseudomonas diminuta, and to test its efficacy in controlling P. coffeae in roots, improving P status in soil and plants, and promoting the growth of Arabica coffee seedlings infested with the nematodes. MHB liquid inoculant was enhanced by optimizing molasses, nitrogen, phosphorus, and MHB concentrations. The five treatments were used, and five replications were in a randomized block-design greenhouse experiment to investigate the AMF Glomus agregatum and MHB inoculant. The improved substrate for MHB liquid inoculant comprised 2% molasses, 0.05% NH4Cl, and 0.1% KH2PO4, with a 2:3 initial volume ratio of B. subtilis and P. diminuta. Scaling up the MHB inoculant in the 2 L reactor boosted the bacterial population to 1010 CFU/mL and the P content to 100 mg/kg. Applying 200 AMF spores and 109 CFU/mL MHB increased leaf number, plant P uptake, and soil P while decreasing root damage and nematode population in soil and roots. Combined AMF and MHB reduced P. coffeae infestation in roots by 70.79% and increased P content in soil and plants by 57.2% and 61.9%, respectively.
Characterization of nitrogen-fixing bacteria isolated from strawberry rhizosphere and their potency to increase seedling growth FEBBY FITRIYANI; REGINAWANTI HINDERSAH; RINA DEVNITA; SYARIFUL MUBAROK; MASAKAZU NAKAYAMA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260445

Abstract

Abstract. Fitriyani F, Hindersah R, Devnita R, Mubarok S, Nakayama M. 2025. Characterization of nitrogen-fixing bacteria isolated from strawberry rhizosphere and their potency to increase seedling growth. Biodiversitas 26: 1964-1974. Nitrogen is vital for plant metabolism and strawberry production. Many farmers heavily rely on inorganic fertilizers to meet their nitrogen requirements, which can have adverse environmental impacts. Nitrogen-Fixing Bacteria (NFB) offer an alternative nitrogen source for sustainable strawberry cultivation. This study aimed to isolate and characterize NFB from potted soil and evaluate their potential to enhance strawberry growth and development. NFB was isolated using nitrogen-free media and characterized based on colony and cell morphology, biochemical properties, and growth-promoting abilities. A total of 21 NFB isolates were obtained from the rhizosphere and bulk soil of six-month-old potted strawberries. The isolates were predominantly Gram-negative and exhibited capsule formation. Eleven isolates underwent detailed biochemical characterization, and three were found to fix nitrogen, produce phytohormones, and synthesize exopolysaccharides. Among these, P. megaterium IA-7 exhibited the highest production of nitrogen (4.23 ?mol mL-1g-1h-1), IAA (31.29 ?g mL-1) and gibberellin (21.73 ?g mL-1), while P. aeruginosa 4A-5 demonstrated superior EPS (4.6 g L-1), and cytokinin production (1.47 ?g mL-1). The in-planta experiment revealed that a 1% inoculant significantly increased leaf number (32.5%) and crown diameter (42.44%) compared to the control treatment. These results underscore that the NFB isolated from the strawberry rhizosphere and bulk soil have promising potential as biofertilizers, offering sustainable alternatives to chemical fertilizers that reduce environmental impact and support eco-friendly farming.
Co-Authors BETTY NATALIE FITRIATIN FEBBY FITRIYANI IIS NUR ASYIAH IMAM MUDAKIR MASAKAZU NAKAYAMA RINA DEVNITA SAON BANERJEE SYARIFUL MUBAROK WIDI AMARIA