AGUS SABDONO
Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro. Jl. Prof. Jacub Rais, Tembalang, Semarang 50275, Central Java, Indonesia

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Antibacterial activity of Gorgonian-associated actinomycetes against diabetic foot pathogens DEVI OKTAVIA ANJANI; ESTER CHRISTIANA CHINTA LOSUNG; AGUS SABDONO; SRI SEJATI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260843

Abstract

Abstract. Anjani DO, Losung ECC, Sabdono A, Sedjati S. 2025. Antibacterial activity of Gorgonian-associated actinomycetes against diabetic foot pathogens. Biodiversitas 26: 4157-4166. Diabetic foot infection is a condition affecting the lower extremities that occurs in at least 15% of all diabetes patients. The treatment of diabetic foot infection has become increasingly challenging due to the risk of infection by microorganisms around the wound. The presence of antibiotic-resistant pathogenic bacteria further aggravates this condition. Therefore, the discovery of new sources of antibiotics is urgently needed. The aim of this study was to explore the antibacterial activity of actinomycetes, particularly those associated with marine gorgonians, to better understand their potential in the treatment of diabetic foot infection. Gorgonian samples were collected from Karimunjawa Waters, Indonesia. Antibacterial activity was screened using agar plug method against Escherichia coli, Staphylococcus aureus, Enterococcus sp., and Proteus sp.. The selected isolates were then identified through Gram staining and molecular identification. The NRPS, PKS-I, and PKS-II genes were detected through the PCR method. The results showed that 12 out of 45 isolates (26.7%) exhibited antibacterial activity against DFI pathogenic bacteria, with four isolates producing inhibition zone greater than 5 mm. The strongest antibacterial activity was exhibited by isolate UR.19.4 and EA.6.5.2 against S. aureus and E. coli with inhibition zones greater than 13 mm. Molecular identification revealed that UR.19.4 and EA.6.5.2 isolates had the closest similarity to Streptomyces griseobrunneus (99.76%) and Streptomyces zhaozhouensis (99.69%), respectively. The NRPS gene was detected in both S. griseobrunneus (UR.19.4 isolate) and S. zhaozhouensis (EA.6.5.2 isolate), while the PKS-II gene was detected only in S. griseobrunneus (UR.19.4 isolate). Therefore, these findings highlight the potential of actinomycetes isolated from Gorgonian as a promising antimicrobial source for treating diabetic foot infections.
Preliminary COI analysis reveals a divergent Carcinoscorpius lineage in North Kalimantan, Indonesian Borneo MOHAMMAD FADNAN AKHMADI; SUTRISNO ANGGORO; AGUS SABDONO; ANINDITIA SABDANINGSIH; DIAH AYUNINGRUM; ASBAR LAGA; ENCIK WELIYADI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 8 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270805

Abstract

Abstract. Akhmadi MF, Anggoro S, Sabdono A, Sabdaningsih A, Ayuningrum D, Laga A, Weliyadi E. 2026. Preliminary COI-based identification of selected horseshoe crab vouchers indicates a divergent Carcinoscorpius mitochondrial lineage in North Kalimantan, Indonesian Borneo. Biodiversitas 27 (8): d270805. https://doi.org/10.13057/biodiv/d270805. Molecular records of Asian horseshoe crabs from North Kalimantan are limited, constraining assessments of taxonomic affinity and mitochondrial variation in Indonesian Borneo. This study examined four morphologically selected voucher specimens from Karis and Mangkudulis islands using mitochondrial Cytochrome C Oxidase subunit I (COI) sequences. The trimmed sequences (653-661 bp) were quality-checked against a common 649-bp homologous region. Phylogenetic and K2P analyses used a 20-sequence, 624-bp alignment, while ASAP and haplotype analyses used the corresponding 19-sequence ingroup matrix. KU1PMTG1 showed 99.85% BLASTn identity to Tachypleus gigas, whereas KU1PKTT4 showed 99.24% identity to T. tridentatus; both assignments were supported by BOLD, low K2P distances, and consistent phylogenetic placement. In contrast, KU1PKCR2 and KU2PKCR3 shared an identical COI haplotype but showed only 91.99% and 91.78% identity to their closest Carcinoscorpius rotundicauda matches. Their K2P divergence from selected C. rotundicauda references ranged from 8.60% to 9.51%, exceeding the 0-1.29% variation within the reference group. The two North Kalimantan sequences formed a strongly supported phylogenetic subclade, a distinct ASAP operational subset, and a haplotype separated by 50 mutational steps from the nearest selected C. rotundicauda haplotype. These concordant patterns indicate a deeply differentiated Carcinoscorpius-affiliated mitochondrial lineage, suggesting potential cryptic diversity or an unsampled regional lineage. However, because this inference is based on two unresolved specimens, one mitochondrial marker, and forward-direction sequencing, they are conservatively retained as putative Carcinoscorpius sp. Broader Borneo sampling, bidirectional sequencing, nuclear markers, and diagnostic morphological comparisons are required to clarify their taxonomic status.
Morphometric and condition analysis of Asian horseshoe crabs from kelong-based fishing encounters in North Kalimantan, Indonesia MOHAMMAD FADNAN AKHMADI; SUTRISNO ANGGORO; AGUS SABDONO; ANINDITIA SABDANINGSIH; RUKISAH RUKISAH; ENCIK WELIYADI; NURIL FARIZAH
Biodiversitas Journal of Biological Diversity Vol. 27 No. 7 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270719

Abstract

Abstract. Akhmadi MF, Anggoro S, Sabdono A, Sabdaningsih A, Rukisah, Weliyadi E, Farizah N. 2026. Morphometric and condition analysis of Asian horseshoe crabs from kelong-based fishing encounters in North Kalimantan, Indonesia. Biodiversitas 27 (7): d270719. https://doi.org/10.13057/biodiv/d270719. Asian horseshoe crabs are conservation-relevant coastal arthropods, but biological baseline information from North Kalimantan, Indonesian Borneo, remains limited. This study analyzed species-specific morphometry, length-weight scaling, body condition, body proportion, and sexual dimorphism of Carcinoscorpius rotundicauda, Tachypleus gigas, and Tachypleus tridentatus. A total of 205 individuals (C. rotundicauda, n: 109; T. gigas, n: 75; T. tridentatus, n: 21) were recorded during 16 fisher-assisted kelong encounters at four coastal stations from January to April 2025. Each individual was identified, sexed, measured, weighed, recorded by station and sampling period, photographed, and released alive after handling. Clear interspecific size differentiation was observed, with T. tridentatus being the largest and heaviest species, C. rotundicauda the smallest, and T. gigas intermediate in size. Species-specific length-weight analysis indicated isometric scaling for T. gigas. The T. tridentatus length-weight estimate was also consistent with isometry but should be considered preliminary, whereas the C. rotundicauda total-length model had low explanatory power and was sensitive to telson condition. Fulton’s condition factor differed among species, while relative condition factor was interpreted mainly within species because it was derived from species-specific equations. Females tended to be larger and heavier than males, but the sex-specific findings for T. tridentatus are preliminary because of its small sample size. Because fishing effort and capture probability were not standardized and repeat encounters could not be excluded, the results describe recorded individuals and should not be interpreted as estimates of abundance, recruitment, catch per unit effort, habitat preference, or population structure. This study provides an integrated morphometric and condition baseline for all three extant Asian horseshoe crab species within a single North Kalimantan kelong-encounter dataset.