Abstract. Suhesti E, Yelmiza, Fitriani D, Hadinoto. 2025. Nutritional profiling of stingless bee bread (Meliponini) across species and sites in Riau, Indonesia. Biodiversitas 26: 6151-6161. Stingless bee bread is a nutrient-dense fermented pollen increasingly promoted as a functional food, yet Indonesian nutritional datasets remain scarce. This study characterizes proximate composition and vitamins A, B2, and E in bee bread from three stingless bee species (Geniotrigona thoracica, Heterotrigona itama, and Trigona apicalis) managed by smallholders at multiple sites in Riau, Indonesia. For each species × site combination, bee-bread pots were pooled to obtain a homogenized composite, which was summarized on a unified fresh-mass basis. Group differences among species and sites were evaluated using non-parametric tests. At a co-occurring site, G. thoracica consistently exhibited the highest levels of vitamins A, B₂, and E; T. apicalis showed the highest total fat and fat-derived energy; and H. itama combined relatively high total energy with elevated protein. Within H. itama, site effects were evident, with vitamins A and E peaking at specific locations and vitamin B₂ at another. These patterns resolve into three practical nutritional signatures, vitamin-oriented in G. thoracica, lipid-oriented in T. apicalis, and relatively stable energy or protein in H. itama. While the pooled composite design constrains colony-level inference, the species- and site-resolved ranges reported here provide preliminary nutritional baselines that can inform future work on quality control and biodiversity-aligned meliponiculture.