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MAHENDRA K. RAI
Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati-444602, Maharashtra, India
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Chemical composition, antimicrobial and antioxidant activity ofessential oils from cumin and ajowan SAHADEO D. PATIL; PANKAJ P. MAKNIKAR; SUSHILKUMAR J. WANKHADE; CHANDRAKIRAN S. UKESH; MAHENDRA K. RAI
Nusantara Bioscience Vol. 8 No. 1 (2016)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n080111

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Abstract. Patil SD, Maknikar PP, Wankhade SJ, Ukesh CS, Rai MK. 2016. Chemical composition, antimicrobial and antioxidant activity of essential oils from cumin and ajowan. Nusantara Bioscience 8: 60-65. Plant essential oils have gained importance as alternative remedies for treatment of many infectious diseases and food preservatives. In the present study, we have determined the chemical composition of the essential oils (EOs) from two Indian spices Cuminum cyminum (cumin) and Trachyspermum ammi (ajowan) of family Apiaceae by gas chromatography-mass spectrometry (GC-MS). Moreover, the antimicrobial potential of these oils was evaluated against several Gram-positive and Gram-negative bacteria using disc diffusion and broth microdilution methods. A Total of 20 major chemical components were analyzed by GC-MS studies and were found to be cuminaldehyde (36.67%) and caren-10-al (21.34%) in case of cumin essential oil while p-cymene (15.54%) and thymol (15.48%) were found to be present in ajowan essential oil. Both the EOs exhibited potent antibacterial effect against most of the tested pathogens. Furthermore, cumin and ajowan EOs demonstrated remarkable antibacterial activity against Salmonella typhi with an inhibition zone diameter of 54 and 60 mm respectively with identical MIC value of 12.5 µl/ml. Ajowan EO was found to exhibit wide spectrum activity against both the Gram-positive and Gram-negative organisms when compared with cumin. Both the essential oils were more potent than standard antibiotic chloramphenicol except cumin against Escherichia coli and Enterobacter aerogenes. Antioxidant activity of cumin was weaker (12.36%) and ajowan was stronger (71.68%) than standard ascorbic acid (20.24%) at 1000 µg/ml concentration when assessed by DPPH radical scavenging assay. Our study suggests that spice essential oils have significant potential in controlling the human and foodborne pathogens.
Determination of ethanol in acetic acid-containing samples by a biosensor based on immobilized Gluconobacter cells ANATOLY N. RESHETILOV; ANNA E. KITOVA; ALENA V. ARKHIPOVA; VALENTINA A. KRATASYUK; MAHENDRA K. RAI
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040301

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Abstract. Reshetilov AN, Kitova AE, Arkhipova AV, Kratasyuk VA, Rai MK. 2012. Determination of ethanol in acetic acid containing samples by a biosensor based on immobilized Gluconobacter cells. Nusantara Bioscience 4: 97-100. A biosensor based on Gluconobacter oxydans VKM B-1280 bacteria was used for detection of ethanol in the presence of acetic acid. It was assumed that this assay could be useful for controlling acetic acid production from ethanol and determining the final stage of the fermentation process. Measurements were made using a Clark electrode-based amperometric biosensor. The effect of pH of the medium on the sensor signal and the analytical parameters of the sensor (detection range, sensitivity) were investigated. The residual content of ethanol in acetic acid samples was analyzed. The results of the study are important for monitoring the acetic acid production process, as they represent a method of tracking its stages.
Tagetes erecta mediated phytosynthesis of silver nanoparticles: an eco-friendly approach UMESH P. DHULDHAJ; SHIVAJI D. DESHMUKH; ANIKET K GADE; MADHU YASHPAL; MAHENDRA K. RAI
Nusantara Bioscience Vol. 4 No. 3 (2012)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n040304

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Abstract. Dhuldhaj UP, Deshmukh SD, Gade AK, Yashpal M, Rai MK. 2012. Tagetes erecta mediated phytosynthesis of silver nanoparticles: an eco-friendly approach. Nusantara Bioscience 4: 109-112. Nanotechnology is a multidisciplinary field having applications in various fields like medicine, pharmacy, engineering, and biotechnology. An important step in nanotechnology is to develop simple and eco-friendly method for the nanomaterial synthesis. Here we describe simple and eco-friendly method for synthesis of silver nanoparticles by extract of Tagetes erecta plant leaves. The phytosynthesis (synthesis by plant) of silver nanoparticles was detected by color change from light-green to dark-brown. Synthesis of silver nanoparticles was confirmed by UV-Vis spectrophotometry, further characterization includes nanoparticle tracking analysis system (NTA) (LM20) and transmission electron microscopy (TEM). TEM analysis confirms the synthesis of the polydispersed spherical silver nanoparticles of 20-50 nm, with an average size of 30 nm.
Co-Authors Alena V. Arkhipova ANATOLY N. RESHETILOV ANIKET K GADE ANNA E. KITOVA CHANDRAKIRAN S. UKESH MADHU YASHPAL PANKAJ P. MAKNIKAR SAHADEO D. PATIL SHIVAJI D. DESHMUKH SUSHILKUMAR J. WANKHADE UMESH P. DHULDHAJ Valentina A. Kratasyuk