ENDANG ANGGARWULAN
Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia

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Extraction and application of coloring agent of shrubby morning glory leaves (Ipomoea carnea subsp. fistulosa) as dye in batik ZULFIYAH ZULFIYAH; ENDANG ANGGARWULAN; SITI LUSI ARUM SARI
Nusantara Bioscience Vol. 7 No. 2 (2015)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n070217

Abstract

Abstract. Zulfiyah, Anggarwulan E, Sari SLA. 2015. Extraction and application of coloring agent of shrubby morning glory leaves (Ipomoea carnea subsp. fistulosa) as dye in batik Nusantara Bioscience 7: 160-165. Natural dye is natural materials whose production is influenced by environmental factor. Natural material content affects the quality and intensity of products’ color. Shrubby morning glory (Ipomoea carnea Jacq. subsp. fistulosa (Mart. ex Choisy) D.F. Austin) has potency as a natural dye since it contains high tannin. The objective of the research is to determine the difference of tannin contents in the extract of shrubby morning glory leaves grow in field or rice field, and also the quality of batik’s color by using dyes extracted from these leaves using fixators iron sulphate (FeSO4), alum (Al2(SO4)3), and calcium oxide (CaCO3). Soil conditions observed are: soil texture, pH, nitrogen content (N), organic materials content, C-organic content. Data of the soil quality are analyzed descriptively. The result of fastness test is analyzed by Kruskal Wallis test and continued by Mann Whitney test with the significance rate of 95%. Data of tannin level are analyzed by t test-independent. The result of the study shows that the leaves of shrubby morning glory from rice-field contain high tannin, which is 1.52%. This type of soil is more fertile than field soil, with better soil texture, pH, N level, organic level, and C-organic. The fastness test shows that using extract shrubby morning glory dye which contains higher tannin is better. Batik’s dyeing using the extract of the plants growing in rice field with fixator of sulfate iron (FeSO4) has the best fastness.
Chitosan treatment and storage temperature in the retardation of fruit ripening of red guava (Psidium guajava) ENDANG ANGGARWULAN; WIDYA MUDYANTINI; ISNA JATI ASIYAH
Nusantara Bioscience Vol. 7 No. 2 (2015)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n070216

Abstract

Abstract. Anggarwulan E, Mudyantini W, Asiyah IJ. 2015. Chitosan treatment and storage temperature in the retardation of fruit ripening of red guava (Psidium guajava). Nusantara Bioscience 7: 153-159. Guava (Psidium guajava L.) is a thin-skinned tropical fruit which is easy to ripe and rot. The objective of the study is to determine the effect of chitosan coating and storage temperature in restraining the ripening of guava and also to the combination of treatments which could be longest to lengthen its storage period. This study uses Completely Randomized Design, with two factors. The first factor is chitosan concentration of 0, 1, 2, and 3%, while the second factor is the storage temperature in 16°C and 28°C with three repetitions during four weeks. The quality of Psidium guajava which is observed during the storage is weight-loss, water content, fruit texture, reducing sugar content, vitamin C content, pigment rind (chlorophyll and carotenoid), respiration rate, and ethylene content. The treatment of chitosan concentration and the storage temperature of 16oC are able to maintain the fruit hardness, vitamin C content, and chlorophyll content; meanwhile, it shows a decrease in weight-loss, reducing sugar, respiration rate, and ethylene content. The combination of chitosan treatment of 3% with a temperature of 16°C is able to maintain the quality and to lengthen the storage period of Psidium guajava up to four weeks.
Application of chitosan spraying on acclimatization success of tiger orchid (Grammatophyllum scriptum) plantlets ARI PITOYO; MUTIA RIZKA HANI; ENDANG ANGGARWULAN
Nusantara Bioscience Vol. 7 No. 2 (2015)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n070222

Abstract

Pitoyo A, Hani MR, Anggarwulan E. 2015.Application of chitosan spraying on acclimatization success of tiger orchid(Grammatophyllum scriptum) plantlets. Nusantara Bioscience 7: 185-191. In Indonesia, tiger orchid or anggrek macan(Grammatophyllum scriptum) is one of the orchid species that have been mass propagatedwith in vitro culture technique. Thesuccessful determinant of in vitro culture after planlet formation is acclimatization.Plantlet have faced with absolutely differentenvironmental condition between inside and outside bottle that potentially severe the survival of theplantlet.The objectives of thisresearch were to know theeffect of chitosan for success of chitosan spraying inacclimatization ofplantlets ofG. scriptumand to knowthe optimum concentration of chitosan that can help thesuccessful of the acclimatization of plantletsG. scriptum.This research used aCompletely Randomized Design (CRD) with four level concentrations of chitosan spraying, there were 0 g/L; 0,5 g/L; 0,75 g/L;1 g/L.Every treatment underwent 15 repetitions. The data taken on this research were qualitative and quantitative data. Qualitative data wasgot from observation of morphology and anatomy then it was analyzed descriptively. The quantitative data was got from percentage ofplantlets life, observation ofshoot length, leaves length, leaves width, number of leaves, number of shoot, index and density of stomatal.The quantitative data was analyzed byAnalysis of Variance (ANOVA), if there was a significant difference between treatment groupsthen followed byDuncan's Multiple Range Test (DMRT) at level significanceof5 %. The result of thisobservation indicated that theeffect of chitosanwassignificant on several parameters including width leaf,plantlets height,and stomatal index while the length andnumber of leaves were not significantly different. The concentration of chitosan real effected on these ranged from 0.5-0.75 g/L.Chitosan concentration at above of 0,75 g/L showed a negative influence on theacclimatization successfully ofplantletsG. scriptum.