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SUTARNO SUTARNO
Bioscience Program, School of Graduates, Sebelas Maret University, Surakarta 57126, Central Java, Indonesia
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Microanatomy alteration of gills and kidneys in freshwater mussel (Anodonta woodiana) due to cadmium exposure FUAD FITRIAWAN; SUTARNO SUTARNO; SUNARTO SUNARTO
Nusantara Bioscience Vol. 3 No. 1 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030105

Abstract

Abstract. Fitriawan F, Sutarno, Sunarto. 2011. Microanatomy alteration of gills and kidneys in freshwater mussel (Anodonta woodiana) due to cadmium exposure. Nusantara Bioscience 3: 28-35. The purpose of this study was to determine the level of Cd accumulation in the gills and kidneys, to know the changes in microanatomic structure of A. woodiana after the various treatments of heavy metals. Completely randomized design pattern of 5 x 3 as used in this laboratory experiment. The amount of exposure of heavy metals Cd was (0 ppm, 0.5 ppm, 1 ppm, 5 ppm, 10 ppm), while the variation of length of exposure time to Cd were (7 days, 14 days, and 30 days). The parameters of Cd accumulation in the gills and kidney was analyzed by using AAS method, while abnormalities of gills and kidney were detected by microanatomy structure. Data collected were then analyzed using the analysis of variance (ANOVA) and continued with further test the DMRT. The results indicated that there is a significant effect in 475.3 > 0.000 and 60150.3 >0.000 with 5% significance level (P<0.05) of Cd treatment on gill and kidney microanatomy of A. woodiana. The changes in microanatomy structure of those organs are including edema, hyperplasia, fusion of lamella, necrosis, and atrophy.
Slaughter weight and carcass of male New Zealand White rabbits after rationing with koro bean (Mucuna pruriens var. utilis) URIP SANTOSO; SUTARNO SUTARNO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010303

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Abstract. Santoso U, Sutarno. 2009. Slaughter weight and carcass of male New Zealand White rabbits after rationing with koro bean (Mucuna pruriens var. utilis). Nusantara Bioscience 1: 117-122. The objectives of the research were to know the effects of koro bean (Mucuna pruriens var. utilis) present on slaughter weight and carcass of rabbits and to know the optimum dosage that resulted in the best slaughter weight and carcass. The research used Randomized Block Design whereas 25 heads of six weeks old rabbits with 450-1270 g of body weight were divided into five groups according to the body weight. Each group was treated with different treatment. The treatment was unpresent of M. pruriens as a control (R0) and various percentage of M. pruriens as much as 21.5%, in the ration with treatment as follows: R1 (raw), R2 (heating), R3 (boiling), and R4 (fermentation). The parameters observed were slaughter weight, carcass weight, meat weight, bone weight, and adipose tissue weight. The data analyzed by analysis of variance (ANOVA) followed with Duncan's Multiple Range Test (DMRT). The presence of  processed M. pruriens could increase production of slaughter weight better than the presence of unprocessed M. pruriens. The additional of 21.5% of fermented M. pruriens resulted in the best production of slaughter weight and carcass of rabbits.
Body weight and statistic vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof AGUS KUNTJORO; SUTARNO SUTARNO; OKID PARAMA ASTIRIN
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010102

Abstract

Abstract. Kuntjoro A, Sutarno, Astirin OP. 2009. Body weight and statistics vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof. Nusantara Bioscience 1: 23-30. This research is aimed to observe the body weight and vital statistic measurement of 50 Texel sheep. Sheep are classified into five treatments of giving woof P0 (giving greenish tree woof without concentrate), P1 (giving greenish woof and concentrate without adding the ramie hay/0%) concentrate), P2 (giving greenish woof and concentrate by adding 10%) ramie hay), P3 (giving greenish woof and concentrate by adding 20%) ramie hay), P4 (giving greenish woof and concentrate by adding 30%) ramie hay), every treatment was repeated 10 times. The result shows that even though it can’t replace the concentrate function, adding ramie hay as much as 10%), 20%) and 30%) on sheep woof can increase the body weight’s growth, respectively 186.67 g/day, 153.34 g/day dan 103.34 g/day. The addition of ramie hay 10%), 20%) and 30%) can increase the addition of statistic vital’s measurement on breast of sheep livestock 1.20 cm); 0.95 cm) and 0.90 cm); the addition of statistic vital measurement on the body length of sheep livestock 0.05 cm); 1.00 cm) and 0.75 cm) and also the addition of breast width is 1.50 cm); 0.15 cm) and 0.3 cm). Meanwhile, the addition of ramie hay on livestock woofs can only increase the addition of vital statistical measurement on the breast at giving 30%) as big as 0.15 cm). It is needed to know further about giving ramie hay by comparing hay of different concentrations of leaf and stems.
Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments SUHARJA SUHARJA; SUTARNO SUTARNO
Nusantara Bioscience Vol. 1 No. 1 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010102

Abstract

Abstract. Suharja, Sutarno. 2009. Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments. Nusantara Bioscience 1: 9-16. This study aims to determine the influence of various fertilization treatments on biomass, chlorophyll, and nitrogen content of leaves from two varieties of chili, Sakti (large chili) and Fantastic (curly chili). The study was conducted in the village of Gatak, Karangnongko sub-district, Klaten District, Central Java, from September 2006 to March 2007. The study used a complete block design with two factorials, chili varieties, and fertilizer treatment. Fertilization treatments includes no fertilizer (control) (P1); manure 2 kg/plant (P2), manure (1 kg/plant) + chemical fertilizer (ZA, SP-36, KCl = 2: 1: 1) + NPK (P3); and manure (1 kg/plant) + chemical fertilizer (SP-36: KCl = 1:1) + liquid organic fertilizer (P4). According to Harborne (1987), chlorophyll content was measured, whereas leaf nitrogen concentration was measured with the Kjeldahl method. Data were analyzed using ANOVA followed by DMRT. The results showed that the Fantastic chili fertilizer treatment affected the biomass and chlorophyll-a but did not affect chlorophyll b, total chlorophyll, and leaf nitrogen. On the curly chili, fertilizer treatment affected fresh plant weight, chlorophyll a, and total chlorophyll but did not affect dry weight, fresh fruit weight, chlorophyll b, and leaf nitrogen. It is, therefore, recommended to use the formulation of manure + chemical fertilizer (SP-36: KCl = 1: 1) + liquid organic fertilizer in the cultivation of chili.
Co-Authors Agus Kuntjoro FUAD FITRIAWAN Okid Parama Astirin SUHARJA SUHARJA SUNARTO SUNARTO URIP SANTOSO