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ADITYA KUSUMAWARDANA
Program of Agronomy, Graduate Program, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Jawa Tengah, Indonesia
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Short Communication: Tetrazolium test for evaluating viability of Capsicum annum seeds ADITYA KUSUMAWARDANA; BAMBANG PUJIASMANTO; PARDONO PARDONO
Nusantara Bioscience Vol. 10 No. 3 (2018)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n100302

Abstract

Kusumawardana A, Pujiasmanto B, Pardono. 2018. Tetrazolium test for evaluating viability of Capsicum annum seeds. Nusantara Bioscience 10: 142-145. Seed quality is important in seeds production. This research was conducted to obtain information of topographical tetrazolium staining pattern of pepper (Capsicum annum) seeds. Tetrazolium test was conducted to determine the seed viability and plant growth vigor. Laboratory test for standard germination and field performances were performed on four lots (A, B, C, D) of pepper seeds. The viability categories pattern were determined by Root Mean Square (RMS), regression, and correlation analyses. Nine topographical patterns were recognized. The laboratory test results and field performances were compared with the topographical pattern. Combination of patterns 1,2 (embryonic axis and cotyledon completely stained) selected as viable category as it gave the least RMS value, the highest determination (R²) and correlation (r) coefficient with standard germination (RMS = 4, 06; R² = 0,761; r = 0,872). Combination of patterns 1,2 also gave the highest determination (R²) and correlation (r) coefficient with field stand (R² = 0,921; r = 0,959). The combination of patterns 1,2 is recommended for estimating plant growth performance in the field.
Co-Authors BAMBANG PUJIASMANTO PARDONO PARDONO