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All Journal Nusantara Bioscience
ARTINI PANGASTUTI
Faculty of Mathematics and Natural Sciences, Graduate Program of Bioscience, Universitas Sebelas Maret. Jl. Ir. Sutami 36A Surakarta 57126, Central Java, Indonesia
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Imidacloprid degradation by potential soil bacteria isolated from rice fields in Grobogan, Central Java, Indonesia MUHAMMAD ALFIYAN HERMAWAN; ARTINI PANGASTUTI; RATNA SETYANINGSIH
Nusantara Bioscience Vol. 16 No. 2 (2024)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n160215

Abstract

Abstract. Hermawan MA, Pangastuti A, Setyaningsih R. 2024. Imidacloprid degradation by potential soil bacteria isolated from rice fields in Grobogan, Central Java, Indonesia. Nusantara Bioscience 16: 284-291. Imidacloprid a widely used pesticide is known for its polar nature, resistance to evaporation, and persistence in soil. When concentrations exceed environmental thresholds, imidacloprid can act as a pollutant, disrupting ecosystems, altering soil pH, and decreasing soil fertility. This study aimed to isolate and identify soil bacteria from rice fields capable of degrading imidacloprid and to highlight their potential role in bioremediation. Isolated bacteria are identified based on morphological characteristics, their ability to degrade imidacloprid and through molecular tests using 16S rRNA. Four bacterial colonies were obtained from the isolation results with different morphological variations. The degradation test results showed that the isolates were able to grow in media containing imidacloprid and were able to reduce imidacloprid by 26.66-31.75%. Based on 16S rRNA gene analysis, isolate IT1 was identified as Enterobacterales, IT2 was identified as the Enterobacteriaceae, IT3 as Pectobacterium aroidearum strain CCRMPA670, and IT4 was identified as Bacillus thuringiensis strain FDAARGOS_791.
Co-Authors MUHAMMAD ALFIYAN HERMAWAN RATNA SETYANINGSIH