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All Journal Indonesian Journal of Ecology and Conservation
Nurmuliayanti Muis
Prodi Pendidikan Biologi, Universitas Sulaewesi Barat, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Diversity and Potential of Nitrogen-Fixing Rhizosphere Bacteria Associated with Foxtail Millet (Setaria italica L.) as Candidates for Biofertilizers in Sustainable Agriculture Yusril Yusril; Nurmuliayanti Muis; Masyitha Wahid; Arlinda Puspita Sari
Indonesian Journal of Ecology and Conservation Vol. 1 No. 2 (2024): Flora and Fauna Conservation
Publisher : Center for Ecology, Conservation and Ethnobiology Studies, Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/ijec.v1i2.6327

Abstract

Foxtail millet (Setaria italica L.) is a potential cereal crop that can be developed as an alternative food source. Rhizosphere microorganisms, particularly nitrogen-fixing bacteria, play an important role in improving soil fertility through nitrogen fixation and the production of plant growth hormones. However, information regarding the potential of rhizosphere bacteria associated with foxtail millet in West Sulawesi remains limited. This study aimed to isolate, select, and identify nitrogen-fixing rhizosphere bacteria associated with foxtail millet and to evaluate their potential as biofertilizer candidates. Bacterial isolation was conducted using a serial dilution method on selective Jensen medium, followed by colony morphology characterization and Gram staining. Selected isolates were further tested for their nitrogen-fixing ability and their capacity to produce plant growth hormones, including indole-3-acetic acid (IAA) and gibberellin (GA₃). Molecular identification was performed using 16S rRNA gene analysis. The results showed that 31 bacterial isolates were successfully obtained from the rhizosphere of foxtail millet, of which 10 isolates were selected based on morphological characteristics. Five isolates demonstrated nitrogen-fixing ability, while three of the best isolates were capable of producing both IAA and gibberellin. Molecular analysis revealed that the best-performing isolates showed high similarity to Bacillus toyonensis and Bacillus cereus. These findings indicate that rhizosphere bacteria associated with foxtail millet have significant potential as biofertilizer candidates to support sustainable agriculture
Species Diversity and Distribution Patterns of Epiphytic Orchids (Orchidaceae) in Bukit Pasapa Kurrak, Mamasa, West Sulawesi, Indonesia Hasgun Hasgun; Nurmuliayanti Muis; Muh Rizal Kurniawan Yunus
Indonesian Journal of Ecology and Conservation Vol. 2 No. 1 (2025): Tropical Ecology and Biodiversity
Publisher : Center for Ecology, Conservation and Ethnobiology Studies, Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/ijec.v2i1.6334

Abstract

Biodiversity refers to the variety of living organisms, encompassing genetic, species, and ecosystem diversity within a particular area. Epiphytic orchids are orchids that grow on other plants but are not parasitic, as they only attach to the trunks, branches, or twigs of living or dead trees without deriving nutrients directly from their hosts. This study aimed to analyze the diversity and distribution patterns of epiphytic orchid species in Bukit Pasapa Kurrak and to explore their implementation as a biological learning resource. This research employed a quantitative approach using an exploratory survey method. Sampling was conducted using a purposive sampling technique. The collected data were analyzed by calculating several ecological indices, including the Important Value Index (IVI), Shannon–Wiener diversity index (H’), Simpson dominance index (C), evenness index (E), and Morisita’s index of dispersion (Id). The results showed that the diversity of epiphytic orchid species in Bukit Pasapa Kurrak was categorized as moderate, with a Shannon–Wiener diversity index of H’ = 1.66. The distribution pattern of epiphytic orchids in the study area was classified as random, with a Morisita index (Ip) of 0.04. Furthermore, validation results from two expert validators indicated that the developed learning resource was highly valid and feasible to implement as a biology learning resource.
Co-Authors Arlinda Puspita Sari Hasgun Hasgun Masyitha Wahid Muh Rizal Kurniawan Yunus Yusril Yusril