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V. A. Parthasarathy
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Agriculture, Biological Sciences & Forestry Library & Information Science

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Cryopreservation of mature coconut embryos by desiccation method Anitha Karun; K. K. Sajini; V. A. Parthasarathy
International Coconut Community Journal Vol 21 No 1 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i01.395

Abstract

Mature embryos of West Coast Tall variety of coconut could be cryopreserved after desiccation pretreatments and retrieved into plantlets. The desiccation pretreatment and optimum duration for dehydration of zygotic embryos were standardized. The maximum retrieval of healthy plantlets was obtained from the embryos subjected to 18 hours silica gel or 24 hours laminar airflow desiccation pretreatment. Irreversible damage caused by desiccation to the death of the shoot meristem was noticed when the water content reduced to 20%.
DNA amplification fingerprinting in coconut: protocol optimization and analysis of resistance to root (wilt) disease K. Jayadev; M. K. Rajesh; K. Devakumar; Regi Jacob Thomas; R. V. Nair; V. A. Parthasarathy
International Coconut Community Journal Vol 21 No 1 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i01.396

Abstract

Root (wilt) disease is a serious malady in coconut causing a loss of approximately 968 million nuts a year in India. Integrated management practices are the only suggested methods to reduce its effect to some extent, while the development of resistant varieties will offer a permanent solution for which concrete breeding efforts are required. Considering the long life cycle of coconut, selection of resistant varieties through conventional methods will be time consuming and laborious. Molecular markers offer numerous advantages over markers traditionally used in plant mapping and selective breeding. Here, we present the optimization of PCR conditions for DNA amplification fingerprinting (DAF) of coconut using arbitrary oligonucleotide primers. Three of the primers could detect variations between root (wilt) resistant and susceptible coconut palms. This study constitutes the basis for future efforts to tag the root (wilt) resistant gene(s) in coconut.
Co-Authors Anitha Karun K. Devakumar K. Jayadev K. K. Sajini M. K. Rajesh R. V. Nair Regi Jacob Thomas