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Standardization And Phytochemical Testing Of Purple Leaf Simplicia (Graptophyllum Pictum) Farah Aulia Azzahra; Heldi Candra
International Journal of Social Science Vol. 6 No. 2 (2026): Agustus 2026
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/ijss.v6i2.13167

Abstract

Background: Purple leaf (Graptophyllum pictum) is a medicinal plant widely used in traditional medicine due to its bioactive compounds, including flavonoids, alkaloids, and steroids. To ensure its safety, quality, and efficacy as a herbal raw material, standardization and phytochemical evaluation are essential. Objective: This study aimed to determine the standardization parameters and phytochemical characteristics of purple leaf simplicia. Methods: The study employed qualitative and quantitative approaches. Purple leaves were cultivated, harvested, processed into simplicia powder, and extracted using the maceration and Soxhlet methods with 96% ethanol. Standardization included moisture content, drying loss, total ash, water-soluble extractive value, ethanol-soluble extractive value, and thin-layer chromatography (TLC) analysis. Data were analyzed descriptively. Results: The cultivation process produced 9 g of simplicia powder. The moisture content, drying loss, total ash, water-soluble extractive value, and ethanol-soluble extractive value were 47.3%, 27.6%, 21.7%, 0.227 g, and 10 g, respectively. Soxhlet extraction yielded 20 mL of concentrated extract. Thin-layer chromatography analysis under UV 254 nm produced Rf values of 0.625, 0.750, and 0.8125, indicating the presence of phytochemical constituents in the extract. Conclusion: Purple leaf simplicia can be successfully standardized using physicochemical parameters and TLC analysis. The findings provide baseline information for quality control and support the utilization of Graptophyllum pictum as a potential raw material for herbal medicine development
Standardization And Phytochemical Test Of Red Betel Leaf Simplicia (Pipper Ornatum) Riza Vetisari; Heldi Candra
International Journal of Social Science Vol. 6 No. 2 (2026): Agustus 2026
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/ijss.v6i2.13168

Abstract

Red betel (Piper ornatum) is a medicinal plant widely utilized in traditional medicine because of its potential pharmacological properties, including antimicrobial, antioxidant, and anti-inflammatory activities. This study aimed to standardize red betel leaf simplicia and evaluate its phytochemical characteristics to determine its quality as a raw material for herbal medicinal products. An experimental laboratory study was conducted using red betel leaves processed into simplicia through harvesting, wet sorting, washing, drying, and grinding. Ethanolic extracts were prepared using maceration followed by Soxhlet extraction. Standardization was performed by evaluating non-specific parameters, including moisture content, drying loss, ash content, water-soluble extractive value, and ethanol-soluble extractive value. Thin-layer chromatography (TLC) and qualitative phytochemical screening were employed to identify the presence of major secondary metabolites. The results showed that several quality parameters complied with the Indonesian Herbal Pharmacopoeia standards, while the moisture content and drying loss exceeded the recommended limits, indicating that the drying process requires further optimization. TLC analysis successfully produced characteristic chromatographic profiles, confirming the presence of bioactive compounds in the extract. These findings suggest that red betel leaf possesses considerable potential as a source of herbal medicine; however, improvements in post-harvest handling and drying techniques are essential to produce standardized simplicia with consistent quality and safety
Preparation Of Simplicia And Testsspecific And Non-Specific Parameters Of Celery Mustard (Cyanthilium Cinereum) Imalia Tiranda; Heldi Candra
International Journal of Social Science Vol. 6 No. 2 (2026): Agustus 2026
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/ijss.v6i2.13169

Abstract

Sky mustard (Cyanthillium cinereum) is a medicinal plant that has long been used in traditional medicine because of its potential pharmacological properties attributed to its bioactive secondary metabolites. Standardization of simplicia is essential to ensure the quality, safety, and consistency of herbal raw materials prior to their utilization in pharmaceutical preparations. This study aimed to prepare sky mustard simplicia and evaluate its specific and non-specific quality parameters as well as its phytochemical characteristics. A laboratory-based experimental study was conducted using sky mustard leaves processed into simplicia through harvesting, wet sorting, washing, drying, grinding, and storage. Ethanolic extracts were prepared using maceration followed by Soxhlet extraction. Quality evaluation included determination of moisture content, drying loss, total ash content, water-soluble extractive value, ethanol-soluble extractive value, and thin-layer chromatography (TLC). The results demonstrated that the water-soluble extractive value (19.1%) and ethanol-soluble extractive value (6%) met the requirements of the Indonesian Herbal Pharmacopoeia. However, the moisture content (18%), drying loss (42%), and total ash content (20%) exceeded the recommended standards, indicating that improvements in the drying and post-harvest handling processes are required. Thin-layer chromatography revealed characteristic chromatographic profiles with Rf values of 0.7125, 0.8150, and 0.9625, confirming the presence of bioactive constituents. These findings indicate that Cyanthillium cinereum has promising potential as a herbal medicinal material, although optimization of simplicia preparation and standardization procedures is necessary to obtain consistent quality and comply with pharmacopoeial standards