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All Journal Jurnal Ilmiah Kesehatan (JIKA)
Arif Mulyanto
Program Studi Teknologi Laboratorium Medik, Universitas Muhammadiyah Purwokerto, Banyumas, Indonesia
Health Professions Medicine & Pharmacology Nursing Public Health

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Optimization of Annealing Temperature and Agarose Concentration in Detecting the mecA Gene of Methicillin-Resistant Staphylococcus aureus (MRSA) Muhammad Abdullah Alhakim; Kurnia Ritma Dhanti; Kurniawan Kurniawan; Arif Mulyanto
Jurnal Ilmiah Kesehatan (JIKA) Vol. 8 No. 1 (2026): Volume 8 Nomor 1 April 2026
Publisher : Sarana Ilmu Indonesia (Salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v8i1.1585

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is associated with the expression of the mecA gene. This study aimed to determine the optimal annealing temperature and agarose concentration to achieve the best PCR product quality based on the evaluation of DNA band integrity, thickness, and resolution. DNA was extracted from five S. aureus samples to detect isolates containing the mecA gene. The experimental variations included annealing temperatures of 53°C, 55°C, 57°C, and 59°C, as well as agarose concentrations of 1% and 1.5%. Through densitometry measurements, the optimal mecA gene band thickness was achieved at an annealing temperature of 59°C. Furthermore, the use of a 1.5% agarose concentration provided the best DNA separation resolution compared to the 1% concentration. In conclusion, optimization of annealing temperature and agarose concentration parameters was crucial to ensure the accuracy of MRSA diagnosis in the laboratory .
Co-Authors Kurnia Ritma Dhanti Kurniawan Kurniawan Muhammad Abdullah Alhakim