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Susiyanti
Sultan Ageng Tirtayasa University
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OPTIMIZATION OF IBA AND ACTIVATED CHARCOAL ON IN VITRO ROOTING OF KOPYOR COCONUT Amanda Putri Viony; Susiyanti; Sulastri Isminingsih; Zahratul Millah; Kartina AM
Gunung Djati Conference Series Vol. 66 (2026): The International Conference on Perennial Crops 2026
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/gdcs.v66i.3402

Abstract

Kopyor coconut (Cocos nucifera L.) is a high-value commodity, but its conventional propagation is constrained by abnormal endosperm, resulting in poor germination. In vitro embryo culture on Y3 medium offers an alternative to improve propagation efficiency. This study evaluated the effects of Indole-3-Butyric Acid (IBA) and activated charcoal on in vitro growth of kopyor coconut embryos cultured on Y3 medium. The experiment used a factorial Randomized Block Design (RBD) with two factors, IBA concentrations (0, 1, 2, and 3 mg L⁻¹) and activated charcoal (0, 1, and 2 g L⁻¹), with four replications. Observed parameters included time to root emergence, explant height, and survival percentage. Data were analyzed using ANOVA followed by Duncan’s Multiple Range Test (DMRT) at a 5% significance level. Results showed that activated charcoal significantly affected time to root emergence and explant height, while IBA and its interaction with activated charcoal were not significant for all parameters. The application of 1 g L⁻¹ activated charcoal produced the fastest root emergence at 9.25 weeks after culture and achieved 100% explant survival. In conclusion, activated charcoal at 1 g L⁻¹ effectively enhances early in vitro development of kopyor coconut embryos by accelerating root emergence and improving survival, while IBA up to 3 mg L⁻¹ does not significantly affect early growth responses.
EFFECT OF BIOSTIMULANT TYPE AND CONCENTRATION ON CIPANAS POTATO (Solanum tuberosum L.) ACCLIMATIZATION Finola Putri Ramadhani; Susiyanti; Sulastri Isminingsih; Zahratul Millah
Gunung Djati Conference Series Vol. 66 (2026): The International Conference on Perennial Crops 2026
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/gdcs.v66i.3411

Abstract

Potato plantlets produced through tissue culture require an acclimatization phase before transfer to field conditions. Natural biostimulants are expected to enhance plantlet adaptation and growth during this critical stage. This study evaluated the effects of biostimulant type and concentration on the acclimatization of Cipanas potato (Solanum tuberosum L.) plantlets. The experiment was arranged in a split-plot design with three replications. The main plot factor was biostimulant type, consisting of coconut water and aloe vera extract, while the subplot factor was biostimulant concentration (0%, 5%, 15%, and 25%). The observed variables included plant height increase, leaf number, and root number. Data were analyzed using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test (DMRT) at the 5% significance level when significant effects were detected. The results showed that biostimulant type, concentration, and their interaction had no significant effect on plant height increase and leaf number during acclimatization. Plant height increase ranged from 0.69 to 2.16 cm, while leaf number ranged from 1.22 to 1.78 leaves plant⁻¹ at 5 weeks after acclimatization (WAA). Root number was the only parameter significantly affected by biostimulant concentration. The control treatment (0%) and 25% concentration produced the highest root numbers, with mean values of 1.56 and 1.16 roots plant⁻¹, respectively, and did not differ significantly according to DMRT. These findings indicate that acclimatization success was primarily influenced by plantlet adaptation to ex vitro conditions, whereas biostimulant concentration contributed mainly to root development.
Co-Authors Amanda Putri Viony Finola Putri Ramadhani Kartina AM Sulastri Isminingsih Zahratul Millah