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AKTIVITAS ANTIOKSIDAN DAN KARAKTERISASI SENYAWA BIOAKTIF DENGAN FTIR PADA EKSTRAK ETANOL Sargassum sp. Elsa Yuliana; Abd. Rohim; Maylina Ilhami Khurniyati; Pinctada Putri Pamungkas; Jihan Novitasari
Prosiding SENTIKUIN (Seminar Nasional Teknologi Industri, Lingkungan dan Infrastruktur) Vol 8 (2025): PROSIDING SENTIKUIN
Publisher : Fakultas Teknik Universitas Tribhuwana Tunggadewi

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Abstract

Sargassum sp. is a brown seaweed species abundantly found along the coastlines of Indonesia. This study aimed to evaluate antioxidant activity and characterize bioactive compounds from ethanolic extracts of Sargassum sp. using Fourier Transform Infrared Spectroscopy (FTIR). The seaweed samples were collected from Kondang Merak Beach, Malang, Indonesia, and extracted by the maceration method using ethanol as the solvent. Variations in the material-to-solvent ratio (1:10 w/v, 1:20 w/v, and 1:30 w/v) and extraction time (20, 40, and 60 minutes) were applied to determine optimal extraction conditions. Antioxidant activity was assessed using the DPPH method, while total phenolic and total flavonoid contents were measured through colorimetric assays using a spectrophotometer. The results showed that the highest antioxidant activity was achieved at an extraction time of 40 minutes with a solvent ratio of 1:10 (w/v), producing an IC₅₀ value of 5.52 mg/mL, which indicates strong free radical scavenging activity. The highest flavonoid content was obtained at a solvent ratio of 1:30 (w/v), reaching 6.54 mg QE/g. Meanwhile, the highest phenolic content was observed at an extraction time of 60 minutes with the same solvent ratio, amounting to 2.58 mg GAE/g. FTIR analysis revealed major functional groups such as O–H, C=O, and C–O, confirming the presence of phenolic and flavonoid compounds that contribute to antioxidant activity. Overall, these findings demonstrate that ethanolic extracts of Sargassum sp. have strong potential as a natural antioxidant source for functional food applications, supported by chemical characterization through FTIR analysis.
Potential of Green Solvent as a Polyphenolic and Antioxidant Extraction Medium in Brown Seaweed Sargassum Cristaefolium Laili Syarifah; Jihan Novitasari; Elsa Yuliana; Maylina Ilhami Khurniyati; Pinctada Putri Pamungkas
SINTA Journal (Science, Technology, and Agricultural) Vol. 7 No. 1 (2026)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.7.1.165-176

Abstract

This study aims to evaluate the effectiveness of water-based environmentally friendly solvents in extracting polyphenol compounds and determine the antioxidant activity of brown seaweed Sargassum cristaefolium. The method used was a quantitative experiment with various types of solvents (aquadest, tap water, and mineral water), temperatures (40°C, 55°C, 70°C), and extraction times (20, 40, 60 minutes). The parameters analyzed included total phenols, total flavonoids, and antioxidant activity using the DPPH method. The results showed that the type of solvent and extraction conditions significantly influenced the release of bioactive compounds. The highest antioxidant activity (79.40%) was obtained from mineral water at 40°C for 60 minutes. The highest phenol content (2.42 mg GAE/g) was also found in mineral water at 70°C, while the highest flavonoid content (4595.42 µg QE/g) was produced by aquadest at 70°C for 60 minutes. In general, increasing extraction temperature and time increases the content of bioactive compounds to a certain limit before thermal degradation occurs. The presence of minerals such as Ca²⁺, Mg²⁺, and HCO₃⁻ in mineral water is thought to increase the stability and solubility of compounds. Thus, water-based solvents have the potential to be a safe and environmentally friendly alternative to organic solvents in the food and healthcare industries.