Aura Apma Patria
Pendidikan Biologi FKIP Universitas Lampung

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ANALISIS PERBEDAAN HASIL FERMENTASI ALKOHOL DENGAN MEDIA WICKERHAM DAN MEDIA ALAMI SARI BUAH Aura Apma Patria; Niluh Putu Sekar Ayu; Nila Oktavia; Bayu Saputra Tri Kusuma; Sumardi; Berti Yolida; Nadya Meriza
Didaktik : Jurnal Ilmiah PGSD STKIP Subang Vol. 12 No. 02 (2026): Volume 12 No. 2, Juni 2026 Release
Publisher : STKIP Subang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36989/didaktik.v12i02.15433

Abstract

This study aimed to analyze the differences in alcohol fermentation results between Wickerham synthetic medium and natural fruit juice medium using Saccharomyces cerevisiae isolates. The research was conducted on April 29, 2026, at the Microbiology Laboratory, University of Lampung, with a one-week anaerobic fermentation period. Ethanol concentration was measured using the oxidation-titration method with K₂Cr₂O₇ solution followed by back-titration using 0.05 N Na-thiosulfate. Results showed that Wickerham medium produced a higher ethanol concentration of 3.04 mg/mL compared to orange juice medium at 2.80 mg/mL. The higher yield in Wickerham medium is attributed to its standardized and complete nutrient composition, particularly the organic nitrogen sources (yeast extract and peptone) that optimally support glycolytic enzyme synthesis required for sugar-to-ethanol conversion. In contrast, the natural fruit juice medium contained unstandardized nutrients, had lower buffering capacity, and potentially contained compounds such as tannins and flavonoids that may inhibit yeast activity. Both media successfully supported anaerobic fermentation as evidenced by the color change of K₂Cr₂O₇ from orange to blue-green. These findings confirm that fermentation medium composition is a critical variable determining ethanol production efficiency by S. cerevisiae.
KAJIAN ETNOSAINS PEMBUATAN IKAN ASIN DI TELUK BETUNG TIMUR UNTUK PEMBELAJARAN IPA KONTEKSTUAL Aura Apma Patria; Rismatul Fadillah; Dwi Kartika Putri; Bayu Saputra Tri Kusuma; Lia Amanda Damayanti; Rani Putri Setiyowati; Berti Yolida; Rini Rita T Marpaung
Didaktik : Jurnal Ilmiah PGSD STKIP Subang Vol. 12 No. 02 (2026): Volume 12 No. 2, Juni 2026 Release
Publisher : STKIP Subang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36989/didaktik.v12i02.15441

Abstract

Effective science education requires learning resources that are relevant to students’ real-life experiences, and one approach that can be used is ethoscience, which integrates local knowledge as a learning medium. However, the application of this approach in the coastal areas of Lampung, particularly in the Teluk Betung region, has not yet been systematically and thoroughly studied. This study employed a descriptive qualitative method, involving four experienced salted fish processors on Pasaran Island, East Teluk Betung, Bandar Lampung City, as research subjects selected through purposive sampling. Data were collected through participant observation, in-depth semi-structured interviews, and documentation, and were then analyzed using the Miles and Huberman model, which includes the stages of data reduction, data presentation, and drawing conclusions. Data validity was ensured through the simultaneous application of source triangulation and methodological triangulation. The research findings reveal that the salted fish production process in the Teluk Betung area involves five main stages: selection of fresh fish, boiling in a salt solution on board the boat, sun-drying, sorting, and packaging of the final product. Each stage incorporates relevant science concepts, including osmosis, sodium chloride ion diffusion, heat transfer (radiation, conduction, and convection), phase changes (evaporation), and mechanisms for inhibiting microbial growth. The unique local practice of boiling fish on a ship involves the concepts of the boiling point of a salt solution and differences in air pressure, which have not been previously reported. This ethoscience study has the potential to be developed into contextual learning tools such as ethoscience-based worksheets and local content teaching modules for Lampung that are relevant to the Merdeka Curriculum.