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All Journal Annales Bogorienses
Puspita Lisdiyanti
National Research and Inovation Agency (BRIN)

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EDITOR'S PREFACE Puspita Lisdiyanti
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.i-vii

Abstract

A Preliminary Report on The Syntheses of Oligonucleotide Primers in The National Research and Innovation Agency (NRIA) Akhirta Atikana; Anggia Prasetyoputri; Yana Rubiyana; Neng Herawati; Desriani Desriani; Riyona Desvy Pratiwi; Dwi Wulandari; Linda Sukmarini; Wien Kusharyoto; Adi Santoso; Masteria Yunovilsa Putra; Puspita Lisdiyanti
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.21-27

Abstract

A PolyGen DNA Synthesizer is equipment that is used for synthesizing oligonucleotide primers for any amplification targets. Oligonucleotide primers are indispensable components for any Polymerase Chain Reaction (PCR)-based detections. In the present study a number of oligonucleotide primer sets were synthesized to target (1) the Human Insuline Glargin (HIG) and (2) the Human Erythropoietin (EPO), as well as (3) the RNA-dependent RNA Polymerase (RdRp) and (4) the Nucleocapsid (N) genes of the severe acute respiratory syndrome virus 2 (SARS-CoV-2). A solid-phase oligonucleotide synthesis method was used according to the default protocol of the Polygen’s instrument to synthesize primers at a 40 nmol scale. The synthesized primers in this study were compared to commercially produced primers in their ability to amplify the gene target(s) in PCR and quantitative real-time PCR (qPCR) reactions. The first two sets of primers showed similar results in PCR compared to commercial primers; however, these primers were not tested for qPCR due to sample limitation. In contrast, the primer sets 3 and 4 were not able to produce amplicons in PCR reactions and only the primer set 4 successfully amplified the gene target in qPCR. These results indicate that the crude primers synthesized in this study are promising candidates for molecular detection and diagnostics, but these primers would benefit from further optimization for routine applications.
Antimicrobial Activities of Actinobacteria Isolated from Marine and Terrestrial Samples in Lombok Island, Indonesia Shanti Ratnakomala; Janina Krause; Miranti Nurindah Sari; Dinihari Indah Kusumawardhani; Mia Kusmiati; Puspita Lisdiyanti
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.28-36

Abstract

During our study on exploring actinobacteria from Indonesian sources, we selected 59 strains of actinobacteria isolated with five different isolation methods from Lombok Island Indonesia. The aim of this research was to isolate and characterize those actinobacteria, and to screen the microbial activities against 5 bacterial tested. Six soil samples from Lombok Island were processed. Totally, 59 isolated were selected. Of these strains, 31 strains showed antibiotic activity. Seventeen strains from 59 strains have been identified by 16S rRNA gene sequencing, and most dominant of the strains belongs to the genus Streptomyces. Three of them were chosen for further experiments due to their broad activity against four of five test strains. From two strains, at least one bioactive substance could be extracted. As only few bioactive substances could be extracted with ethyl acetate from liquid cultures more experiments should be performed on this subject. More solvent extraction should be tested as well as other production media. In conclusion, present study did now reveal bioactive actinobacteria were isolated from soil samples in Lombok Island. Antimicrobial compound has also been detected which is active against a broad range of pathogens. These findings may have an importance to produce potentially new antibiotic substances from actinobacteria indigenous Indonesia.
EDITOR'S PREFACE Puspita Lisdiyanti
Annales Bogorienses Vol. 26 No. 2 (2022): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Co-Authors Adi Santoso Akhirta Atikana Anggia Prasetyoputri Desriani Desriani Dinihari Indah Kusumawardhani Dwi Wulandari Janina Krause Linda Sukmarini Masteria Yunovilsa Putra Mia Kusmiati Miranti Nurindah Sari Neng Herawati Riyona Desvy Pratiwi Shanti Ratnakomala Wien Kusharyoto Yana Rubiyana