A digital herbarium is a herbarium that has been digitized using technology to convert information from conventional data into digital form. This research study uses the concept of a digital herbarium based on real photos from school plant inventories and equipped with metadata on scientific names and morphological characteristics integrated in the form of a website. The purpose of this study is to develop technology-based learning media to improve students' understanding of abstract concepts of biodiversity. This study was conducted using an R&D method, with a one-group pretest-posttest design implemented during the product trial. The research instruments used multiple-choice comprehension test questions and expert validation sheets. The results of the study obtained the final form of the website containing 21 plant species from 17 families. The digital herbarium website learning media is classified as highly valid based on expert media validation results of 88%, subject matter expert validation of 96%, and student response of 84%. Based on expert validation, the developed product was declared valid for use in learning. In addition, the t-test analysis showed a significant difference between pretest and posttest scores (p = 0.001 < 0.05), which indicates the effectiveness of the product in enhancing students’ learning outcomes. The results indicate that the digital herbarium website learning media effectively improved students’ understanding of biodiversity material, with an N-Gain score of 0.63 in the moderate category. Based on the results of the study, the digital herbarium website learning media was valid and effective in improving students’ understanding of biodiversity material in biology learning.