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Antibacterial Activity of Papaya Fruit (Carica papaya L) Ethanol Extract Against Escherichia coli Jendri Korisano; Ivonne Mona Selly Panjaitan
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11646

Abstract

The increasing occurrence of antibiotic resistance highlights the critical necessity to investigate other antibacterial options, especially those originating from natural materials. Papaya (Carica papaya L.) is a tropical species recognized for having multiple bioactive substances that may exhibit antibacterial properties. The study aimed to assess the antibacterial activity of papaya fruit pulp extract at concentration of 40%, 50% and 60% against Escherichia coli with four replications. The extract was prepared using an appropriate extraction technique and evaluated through an in vitro antibacterial assay. Antibacterial activity was determined by measuring the inhibition of bacterial growth in comparison with a control group. Findings revealed that papaya fruit pulp extract exhibited strong inhibitory effects against Escherichia coli. There was no significant difference between concentration (p value = 0.83). The result indicates that papaya fruit pulp may represent an alternative source of antibacterial compounds and support its further development for antimicrobial applications. This study expands the scientific understanding of papaya fruit pulp as a promising natural antibacterial resource.
Antibacterial Activity of Red Algae Extract (Eucheuma cottonii) Against Propionibacterium acnes and Staphylococcus epidermidis Bacteria Andreas Lapu Dwitama Bongga; Ivonne Mona Selly Panjaitan; Joshua H. L. Tobing
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11997

Abstract

Acne is a frequent skin issue characterized by inflammation, linked to an overproduction of oil and the growth of bacteria, especially Propionibacterium acnes and Staphylococcus epidermidis. This study aimed to evaluate the antibacterial activity of red algae (Eucheuma cottonii) extract at concentrations of 25%, 35%, and 45% against these acne-causing bacteria. The extraction process utilized the maceration technique with 96% ethanol over a period of 72 hours. The evaluation of antibacterial activity was performed through the Kirby–Bauer disk diffusion technique. The results demonstrated that all tested concentrations produced inhibition zones classified as moderate against both bacteria. The highest antibacterial activity was observed at the 45% concentration, with mean inhibition zones of 7.3 ± 0.87 mm for P. acnes and 8.2 ± 1.67 mm for S. epidermidis. Statistical analysis using one-way ANOVA indicated no significant differences in antibacterial activity among the tested concentrations for both P. acnes shown p value is .171 (p > 0.05) and S. epidermidis shown p value is .053 (p > 0.05). These findings suggest that E. cottonii extract possesses moderate antibacterial activity and has potential as a natural active ingredient for the development of anti-acne topical formulations, although increasing concentration within the tested range does not significantly enhance its effectiveness.