The coffee business in Pupuan has great potential, farmers face several challenges, namely workloadand worker fatigue which are often the main causes of work accidents. Coffee processing workers atUD. Cipta Lestari stated that they experienced back pain, arm pain, and joint and muscle problems.Based on these problems, this study aims to determine the level of workload and fatigue for workersand identify strategies that can reduce worker fatigue in processing coffee at UD Cipta Lestari PupuanBali. The research method used is a quantitative method with a survey. The population in this studywere 11 workers and the sample was the 10 people (Purposive Sampling). Assessment of physical andmental fatigue using the Fatigue Assessment Scale (FAS) questionnaire method. Mental workloadassessment using the National Aeronautics and Space Administration Task Load Index (NASA-TLX)method and using physical workload measurements with Cardiovascular Load (CVL) calculations. Theresults of this study indicate that the score on the calculation of physical workload with an average CVLshows a figure of 9.95% and for the mental workload score with the NASA-TLX questionnaire reaches67.5 with a high mental workload category. The average FAS questionnaire for worker physical fatigueis at 2 based on questions 1-5 with the interpretation of "sometimes". The average mental fatigue is at 2from questions 6-10 which also shows the interpretation of "sometimes". Thus, from the results of thisstudy it is concluded that workers at UD Cipta Lestari Pupuan Bali have a high mental workload andworkers also feel tired but not continuously.