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All Journal Horizon: Indonesian Journal of Multidisciplinary
Visca, Annisa Septania
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Agriculture, Biological Sciences & Forestry Humanities Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Languange, Linguistic, Communication & Media Social Sciences Other

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Teknik Pengambilan Darah dan Pemisahan Serum Darah Sapi pada Sampel Untuk Uji Bovine Viral Diarrhea (BVD) Visca, Annisa Septania; Azzahra, Intan Apriyola Ropi; Putra, Naufal Anugerah; Amanda, Norma Desti
HORIZON: Indonesian Journal of Multidisciplinary Vol. 4 No. 4 (2026): HORIZON: Indonesian Journal of Multidisciplinary (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/hijm.v4i4.6883

Abstract

Bovine Viral Diarrhea (BVD) is an infectious disease in cattle that can cause reproductive disorders, decreased productivity, and economic losses. The success of BVD examination is greatly influenced by the quality of blood serum used as a laboratory test material. This study aims to describe the blood collection technique and serum separation procedure for cattle and evaluate the quality of the resulting serum to support BVD laboratory examination. Sampling was conducted in July–August 2025 in seven districts in Bengkulu Province through the jugular vein, auricular vein, and coccygeal vein. Blood samples were collected using syringes or vacuum tubes without anticoagulant, then allowed to clot for 24 hours in an inclined position before the serum was separated and centrifuged at 3500 rpm for 10 minutes. The obtained serum was stored at −20°C until inactivated at 56°C for 30 minutes. The results showed that the serum collection and separation procedures produced clear serum, free from hemolysis, and suitable for use as a BVD laboratory test material. Thus, proper implementation of serum collection, separation, storage, and inactivation procedures plays an important role in ensuring sample quality and supporting the reliability of BVD examination
Uji Kualitatif Kandungan Parasetamol pada Jamu Pegal Linu dengan Metode Kromatografi Lapis Tipis (KLT) Azzahra, Intan Apriyola Ropi; Saraan, Sri Muftri Diani; Hilmi, Anisa Prima; Putra, Naufal Anugerah; Visca, Annisa Septania
HORIZON: Indonesian Journal of Multidisciplinary Vol. 4 No. 4 (2026): HORIZON: Indonesian Journal of Multidisciplinary (In-Press)
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/hijm.v4i4.6959

Abstract

Herbal medicine for muscle and joint pain is a traditional medicine widely used by the public to help reduce muscle and joint pain. However, the potential addition of chemical drugs (BKO), such as paracetamol, is still a concern because it can pose health risks if consumed without supervision. This study aims to identify the presence of paracetamol in herbal medicine samples using the Thin Layer Chromatography (TLC) method. The research samples consisted of several herbal medicine preparations obtained from the market and were analyzed qualitatively by comparing the characteristics of sample spots to the paracetamol reference standard based on the retention factor (Rf) value and visualization under 254 nm UV light. The analysis results showed that the Rf value of the paracetamol standard in the test system used was within a certain range, while the analyzed herbal medicine samples did not show spots that were parallel to the reference standard. Based on these results, all tested samples were declared negative for the presence of paracetamol. This study shows that the TLC method can be used for the initial identification of the presence of paracetamol in herbal medicine samples in the scope of the qualitative testing conducted.
Co-Authors Amanda, Norma Desti Azzahra, Intan Apriyola Ropi Hilmi, Anisa Prima Putra, Naufal Anugerah Saraan, Sri Muftri Diani