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All Journal Indonesian Journal of Agricultural Research Akta Agrosia Jurnal Pengabdian Masyarakat
Inoriah, Entang
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Journal : Akta Agrosia

 1 
Optimalisasi PCR-RAPD dan Identifikasi Morfologi Tanaman Kumis Kucing di Provinsi Bengkulu Marulak, Simarmata; Inoriah, Entang; Haquarsum, Eka Jan Virgin
Akta Agrosia Vol 17 No 2 (2014)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

A research was conducted with objectives to look for the best DNA extraction methods for whisker plants, to optimize random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR) by testing annealing temperatures and screening random primers, and to identify morphology of these plants in Bengkulu Province. DNA’s extraction followed the protocols of Geneaid and Epicentre kits were conducted for 11 clones of whisker plants. Optimalization of RAPD-PCR was tested by six levels of annealing temperatures and 16 random primers. Morphological identifications were done in 4 locations in Bengkulu Province, i.e. Bengkulu City, Kuro Tidur (North Bengkulu), Kepahiang, dan Air Dingin (Curup)  Results showed that Geneaid demostrated better results as compared with Epicentre.  RAPD-PCR performed optimum at 34.8 oC of annealing temperature; whereas, three random primers showed optimal amplification were I-01, N-01 and P-01, which nucleotide sequences were ACCTGGACAC, CTCACGTTGG, and GTAGCACTCC, respectively.  Meanhile,  no morphological variatons was found among the the samples, except that growths of whisker plants were taller and bigger in Kepahyang and Air Dingin Curup than in others.
Pematahan Dormansi Benih Kebiul (Caesalphinia bonduc L.) dengan Berbagai Metode Uyatmi, Yesi; Inoriah, Entang; Marwanto, Marwanto
Akta Agrosia Vol 19 No 2 (2016)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Seed of Caesalpinia bonduc L. is difficult to germinate due to the thick and hard seed coat. The research aims to evaluate various methods for breaking dormancy of C. bonduc seed. This study was conducted from December to January 2016 The way to break the dormancy were arranged in Completely Randomized Design. Eleven methods to solve the seed dormancy of C. bonduc were compared. The ways to break the dormancy were soaking in water with different temperatures. The temperatures were 30C for 0 hours, 5 hours, 10 hours, and 15 hours. Other techniques to break the dormancy were soaking in hot water at 10000C for 5 hours, 10 hours, and 15 hours. Warm stratification with 100% air humidity (RH) at 40C for five days, ten days and 15 days also included as treatments. The last method was seed piercing with a needle. The variables observed in this study were germination capacity, germination rate, epicotyl length, plant height, and root length. The results of this study showed that the 11 methods of breaking seed dorman cy significantly affected germination capacity, germination rate, emerging epicotyl, and plant height. Seed piercing classified as the most efficient method as indicated by the value of 100% in germination capacity, 1.43 in germination rate, 13.64 cm in plant height, and 6.16 cm in root length.0
Growth and Yield Response of Melon to Different Time Application of Goat Manure and Dose of Potassium Handajaningsih, Merakati; Marwanto, Marwanto; Armitasari, Tri; Inoriah, Entang; Pujiwati, Hesti
Akta Agrosia Vol 21 No 1 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Goat manure contains high potassium, thus it is expected to reduce  the usage of synthetic potassium fertilizer. Incubation of goat manure is needed prior to planting since the nutrients are slow release. The objective of the experiment was to find the best time application and dose of potassium on growth and yield of melon. Container experiment using polybags was set in Completely Randomized Design.  Goat manure were incorporated into soil at the time of transplanting (0 DBT), 5 days before transplanting (5 DBT), and 10 DBT. The dose of potassium was consisted of 0 kg/ha, 200 kg/ ha and 400 kg/ha. The treatments were repeated 3 times, each of which consisted of 4 plants. Growth and yield of plants showed no response to the interaction between the two factors of treatment. Single effect of time applicaton of goat manure 10 DBT affected leaf area (1619.51 cm2), fruit weight (872 g), fruit diameter (16.19 cm) and the thickness of edible fruit part (2.61 cm). The dose of potassium 400 kg/ha increased fruit sweetness to 8.20Brix.
Co-Authors Annisa Fauziah, Annisa Armitasari, Tri Haquarsum, Eka Jan Virgin HESTI PUJIwATI Marulak, Simarmata Marwanto Marwanto, Marwanto Merakati Handajaningsih Prasetyo Prasetyo Priambodo, Andrian Uyatmi, Yesi Wuri Prameswari