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All Journal Jurnal Biologi Tropis
Faustina De Yesu Prisila Abi
Catholic Widya Mandira University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Impact Sugar Type, Fermentation Duration, and Concentration of Eco-Enzyme on pH, TDS, and Antibacterial Activity Ni Putu Vidya Primarista; Damiana Nofita Birhi; Faustina De Yesu Prisila Abi; Fena Retyo Titani; Arya Wiranata; Abdul Rohman Wali
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12651

Abstract

Eco-enzymes produced from fruit and vegetable waste are increasingly used for waste valorization and natural disinfection, but the combined effects of sugar type, fermentation duration and dilution on their quality remain unclear. This study examined eco-enzymes from mixed fruit peels prepared with granulated sugar, brown sugar and molasses, fermented for 1, 2 and 3 months, and diluted to 20%, 10%, 1%, 0.2% and 0.1%. Physicochemical properties (pH, TDS) and antibacterial activity against Staphylococcus aureus and Escherichia coli were measured; data were analysed by one-way ANOVA with Tukey’s test and Pearson correlation. Eco-enzymes were strongly acidic (≈3.5–3.9) at 20–10% and approached neutrality at 0.1%, with molasses-based formulations maintaining the lowest pH at high dilutions. TDS showed fermentation‑time‑dependent trends that differed between concentrated and dilute solutions. No inhibition was observed in month 1; activity appeared after ≥2 months and was highest at 3 months, with three‑month molasses eco-enzymes at 20% giving the largest inhibition zones and retaining activity at 0.1–0.2%. These findings identify molasses and extended fermentation as key levers to improve eco-enzyme performance as low-cost natural antibacterial agents.
Co-Authors Abdul Rohman Wali Arya Wiranata Damiana Nofita Birhi Fena Retyo Titani Ni putu Vidya Primarista