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All Journal PROCEEDING ICTESS (Internasional Conference on Technology, Education and Social Sciences)
Pairote Wongputtisin
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Computer Science & IT Education Social Sciences

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SCREENING PHOSPHATE-SOLUBILIZING BACTERIA FROM RHIZOSPHERE SOIL UNDER DURIAN (DURIO ZIBETHINUS L.) CANOPIES IN THAILAND Apinya Namnon; Sawika Konsaeng; Pairote Wongputtisin; Jiraporn Inthasan
PROCEEDING ICTESS (Internasional Conference on Technology, Education and Social Sciences) Vol 7 (2026): ICTESS 7th
Publisher : Universitas Slamet Riyadi Surakarta

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Phosphate-solubilizing bacteria (PSB) play a crucial role in enhancing phosphorus availability in agricultural soils. This study aimed to isolate and screen efficient PSB from rhizosphere soils under durian (Durio zibethinus L.) orchards in eastern and southern Thailand. A total of 40 bacterial isolates were obtained and evaluated for phosphate-solubilizing activity using Czapek’s and Pikovskaya’s medium. Three isolates (R24, J33 and J35) exhibited the highest solubilization efficiency. Isolate R24 produced the largest clear zone on Czapek’s medium (1.30 ± 0.33 cm), followed by J33 (1.10 ± 0.03 cm); meanwhile, J35 showed the highest activity on Pikovskaya’s medium (0.60 ± 0.14 cm). Molecular identification based on 16S rRNA gene sequencing revealed that isolates R24 and J35 were closely related to Pseudomonas orientalis, whereas J33 was identified as Massilia norwichensis. These findings highlight the potential application of selected PSB as biofertilizers to improve phosphorus availability and support sustainable durian cultivation in Thailand. Keywords: phosphate-solubilizing bacteria, durian, Thailand
ISOLATION, SCREENING, AND IDENTIFICATION OF POTASSIUM-SOLUBILIZING MICROORGANISMS FROM RUBBER PLANTATION SOILS IN PHITSANULOK PROVINCE Pradubphong Saenbutra; Sawika Konsaeng; Chackapong Chaiwong; Pairote Wongputtisin; Jiraporn Inthasan
PROCEEDING ICTESS (Internasional Conference on Technology, Education and Social Sciences) Vol 7 (2026): ICTESS 7th
Publisher : Universitas Slamet Riyadi Surakarta

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Potassium-solubilizing microorganisms have potential for improving potassium availability in agricultural soils, particularly in rubber plantations where a large proportion of soil potassium occurs in mineral forms that are not readily available to plants. This study aimed to isolate, screen, and identify native potassium-solubilizing microorganisms from rubber plantation soils in Wang Thong district, Phitsanulok province, Thailand. Rhizosphere soil samples were collected from rubber plantations managed under organic and chemical fertilizer systems. A total of 48 microbial isolates were obtained using Aleksandrov agar medium supplemented with mica as an insoluble potassium source. Among these isolates, 28 produced clear halo zones, indicating their ability to solubilize potassium from an insoluble mineral source. Based on radial clear zone formation, colony characteristics, and representation of sampling sources, 12 promising isolates were selected for quantitative evaluation in Aleksandrov liquid medium. After 14 days of incubation, soluble potassium concentrations ranged from 3.01 to 5.50 mg L⁻¹. The highest concentrations were observed in isolates No. 1, No. 10, and No. 11, with values of 4.93, 5.31, and 5.50 mg L⁻¹, respectively. These isolates were identified by 16S rRNA gene sequence analysis as Bacillus siamensis, Pseudomonas orientalis, and Bacillus subtilis. The results indicate that rubber plantation soils in Phitsanulok province harbor native potassium-solubilizing microorganisms with potential for further development as potassium-solubilizing biofertilizer candidates. Keywords: potassium-solubilizing microorganisms, rubber plantation soil, Bacillus siamensis, Pseudomonas orientalis, Bacillus subtilis, biofertilizer
Co-Authors Apinya Namnon Chackapong Chaiwong Jiraporn Inthasan Pradubphong Saenbutra Sawika Konsaeng