Article Per Year (5 Year)
p-Index From 2020 - 2025
1.037
P-Index
This Author published in this journals
All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Ulum, M. F.
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
Prediction of meat quality in Bali cattle using ultrasound imaging Jakaria, J.; Khasanah, H.; Priyanto, R.; Baihaqi, M.; Ulum, M. F.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 2 (2017): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.2.59-65

Abstract

The objective of this study were to predict carcass quality characteristics in Bali cattle using ultrasound imagery. The Number of samples were 81 heads of Bali cattle consist of bulls (62 heads) and cows (19 heads) with various age ranging from 1 to 6 years were collected their body weight and carcass qualities including backfat thickness (BF), longissmus dorsi thickness (LD), rump fat thickness (RF), rump thickness (RT), marbling score (MS) and the percentage of intramuscular fat (PIF). Those were estimated using ultrasound performed on 4.5-6,5 MHz frequency with depth of 8.8-13 cm. The BF, LD, MS and PIF measurement were applied on 12th-13th ribs, while the RT and RF measurement were conducted between ischium and illium. MS determination was calculated using Aus-Meat standard, while PIF was analysis based on Deaton and Rouse (2000). Body weight and carcass quality among traits were analyzed using descriptive and correlation procedures. The results showed that performance of body weight and carcass quality differs between Bali bulls and Bali cows, as well as among the age variations. Correlation analyses among traits (body weight and carcass quality) showed strong positive correlation (P<0.05) ranging from 0.291 to 0.938. In conclusion, ultrasound imaging method could be used to estimate carcass quality characteristics in Bali cattle.
Association of SNP g.643G>A of MYF5 gene polymorphism with body weight and body measurements in Bali cattle Saputra, E. A.; Ulum, M. F.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 1 (2020): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.1.1-6

Abstract

This study aimedto identify the SNP g.643G>Aof MYF5 genethen associate it with body weight and body size measurements in Bali cattle. Blood samples were collectedfrom 80 bali cattle at BPTU-HPT Denpasar Bali. Data on phenotypic properties observed included; birth weight, live weight, average daily gain, body length, chest depth, withers height, hip height, and heart girth. Polymorphismof the MYF5 gene was identifiedusing the PCR-RFLP method. Association of MYF5 genotypes with body weight and body size measurementswas performed using General Linear Model by SAS 9.4 program. MYF5│MspI gene was polymorphic with three genotypes: AA, AG, and GG. Genotype frequency of AA, AG and GG were 0.04 (3), 0.30 (24), and 0.65 (53), respectively. Allele frequencies were 0.19 and 0.81 for A and G alleles, respectively. Gene frequency analysis showed that Bali cattle at BPTU-HPT Denpasar was in Hardy-Weinberg Equilibrium. Association of MYF5│MspI gene with body weight and body measurement were not significantly different. SNP g.643G>A could not be used as a genetic marker for the body weight and body size measurements in Bali cattle.
A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle Dairoh, D.; Jakaria, J.; Ulum, M. F.; Sumantri, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 1 (2022): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.1.17-28

Abstract

The objective of this study was to evaluate single nucleotide polymorphisms (SNP) in the 3'UTR region of CAPN1 gene by direct sequencing technique in beef cattle and its influence on growth and meat quality of the contrast mode ultrasound image in Bali cattle. The numbers of beef cattle used were Bali cattle (n=52 heads) which compared to purebred Belgian Blue (n=30 heads), Limousine (n=14 heads), Wagyu (n=7 heads), and PO (n=10 heads). Genetic diversity data were obtained from calculations by PopGen 1.32 software. Ultrasound imaging of the thoracic longissimus dorsi muscle in Bali cattle was carried out between the 12 − 13 ribs, and the sonogram was analyzed by Image-J NIH software. The result shown that 3’UTR of CAPN1 gene was found in six discovery SNPs that polymorphic in Bali cattle, they were g.15284 C>T, g.15347 T>G, g.15525 G>A, g.15853 G>A, g.15905 G>A, g.15915 G>A and Indel mutation was polymorphic in Bali cattle, Belgian Blue, Limousine, and PO. There was no association between these SNPs and growth traits. However, SNP g.15525 G>A was significantly associated (P<0.05) with a backfat thickness (BFT) in Bali cattle. In conclusion, the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS) related to meat quality.
A Novel SNPs of the SREBF1 and SCARB1 Genes and the Association with Fatty Acid Profile in Bali Cattle Dairoh; Ulum, M. F.; Jakaria; Ishak, A. B. L.; Sumantri, C.
Tropical Animal Science Journal Vol. 46 No. 4 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.4.428

Abstract

This study aimed to investigate the genetic impact of single nucleotide polymorphisms (SNPs) of the sterol regulating element binding factor 1 (SREBF1) and scavenger receptor class B member 1 (SCARB1) genes on carcass and meat characteristics, as well as fatty acid composition, in the Bali cattle. The blood and beef samples used for DNA sequencing, physical assessment, and fatty acid analysis were collected from 95 male Bali cattle. The ultrasound images were analyzed using the Image-J NIH software. A total of 4 SNPs were identified in the SREBF1 gene and 5 SNPs in the SCARB1 gene. The results showed that the 4 SNPs in the SREBF1 gene, namely g.12629T>C, g.12731T>C, g.12881A>G, and g.12986C>T, were associated with heptadecanoic acid (C17:0) and cis-11-eicosanoic acid (C20:1). The SNPs g.12731T>C of the SREBF1 gene was associated with fat content, palmitoleic acid (C16:1), stearic acid (C18:0), cis-11-eicosanoic acid (C20:1), and total fatty acids. Furthermore, 4 SNPs in the SCARB1 gene, including g.72219C>T, g.72380C>A, g.72517G>A, and g.72607C>T correlated with longissimus dorsi thickness (LDT). All SNPs in the SCARB1 gene showed significant associations with cis-10 heptadecanoic acid (C17:1) and cis 8,11,14-eicosatrienoic acid (C20:3n6). The SNP g.72400A>G of the SCARB1 gene was related to caprylic acid (C8:0), lauric acid (C12:0), arachidonic acid (C20:4n6), monounsaturated fatty acids (MUFA), and unsaturated fatty acids (UFA). These results suggested that the identified polymorphisms in the SREBF1 and SCARB1 genes could serve as valuable references for investigating similar genes in other cattle breeds, particularly concerning fatty acids.
Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle Kholijah, K.; Darwati, S.; Ulum, M. F.; Londra, I. M.; Noor, R. R.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 4 (2024): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.4.297-306

Abstract

Cattle coat color is governed by numerous genes, notably the tyrosinase gene (TYR). This study analyzed coat color anomalies like albinism and white spotting in Bali cattle. It aims to discern the TYR gene's diversity, expression patterns, and correlation with coat color abnormalities. The research encompassed 189 cattle, including those with standard coat color (n=53), white-spotted (n=11), and albino (n=17) Bali cattle, as well as Simmental (n=37), Limousin (n=14), Madura (n=21), and Peran-akan Ongole (PO) cattle (n=36). Total DNA was extracted and the TYR gene in exon 1 was amplified using forward and reverse primers with a target amplicon length of 994 bp. Direct sequencing unveiled TYR gene diversity, analyzed using BioEdit and MEGA6 software to identify SNPs. PCR-RFLP was used for SNP genotyping, while qPCR analyzed TYR gene expression. Two mutations (SNP g. 939A>G and SNP g. 887C>T) were discovered in Bali cattle TYR exon 1. SNP g. 939A>G exhibited polymorphism, with the highest GG genotype frequency in standard Bali cattle, indicating a high G allele frequency. Conversely, Madura, Simmental, Limousin, and PO cattle had the lowest allele fre-quency. Chi-square (χ² test) results showed non-significance across all cattle types. TYR gene expres-sion differed significantly between standard Bali cattle and albinos (p<0.05).
SNP Detection in FREM2 Gene and Its Association with Carcass Quality in Bali Beef Pertiwi, E. A.; Ulum, M. F.; Jakaria, J.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.149

Abstract

The FRAS1-related extracellular matrix protein 2 (FREM2) gene is one of the genes that play a role in controlling marbling scores in beef cattle. This study aimed to identify SNPs in exon 6 of the FREM2 gene and its association with carcass quality in Bali beef using ultrasonography. A total of 93 cattle were used: 55 cattle from Banjarmasin slaughterhouse, South Kalimantan, Indonesia, 28 cattle from Bali Cattle Breeding Centre in Bali Province, and 10 cattle from UPTD Kupang Regency, NTT, Indonesia. SNP of the FREM2 gene was identified by using sequencing techniques and then genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The association of FREM2 gene SNPs with carcass characteristics was analyzed using the General Linear Model (GLM) method using SPSS software version 22. Carcass (longissimus dorsi thickness; back fat thickness) and meat (intramuscular fat; marbling score) characteristics were identified non-invasively using ultrasonography images and then analyzed with Image-J NIH software. SNP at position g.89327G>A was located in exon 6 of the FREM2 gene and did not change the amino acid (prolineproline) sequence in Bali beef. The genotyping results with PCR-RFLP technique SNP g.89327G>A FREM2|BccI gene has high diversity. The alleles of A and G were 0.747 and 0.253, respectively. The diversity of SNP g.89327G>A was significantly associated (p<0.05) with longissimus dorsi thickness (LD), while back fat thickness, intramuscular fat, and marbling score did not significantly different (p>0.05). Thus, SNP at position g.89327G>A in exon 6 of the FREM2 gene might be used as a candidate genetic marker for carcass quality in Bali beef.
Co-Authors A. B. L. Ishak, A. B. L. C. SUMANTRI Dairoh Dairoh, D. Jakaria Jakaria Khasanah, H. Kholijah, K. Londra, I. M. M. Baihaqi Pertiwi, E. A. R. Priyanto R. R. Noor, R. R. S. Darwati Saputra, E. A.